| Journal: | Brazilian journal of veterinary research and animal science |
| Database: | PERIÓDICA |
| System number: | 000291643 |
| ISSN: | 1413-9596 |
| Authors: | Martins, Ana Maria Cristina Rabello Pinto da Fonseca1 Tamaso, Elia2 Guerra, Jose Luiz3 |
| Institutions: | 1Instituto Biologico, Laboratorio de Anatomia Patologica, Sao Paulo. Brasil 2Universidade de Sao Paulo, Faculdade de Medicina Veterinaria e Zootecnia, Sao Paulo. Brasil 3Universidade de Sao Paulo, Departamento de Patologia, Sao Paulo. Brasil |
| Year: | 2002 |
| Volumen: | 39 |
| Number: | 1 |
| Pages: | 43-49 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental |
| English abstract | The aim of the present study was to study some of the proteins that form the extracellular matrix of 54 benign and malignant mammary neoplasms in dogs, using histochemical methods: Picrosirius and polarization microscopy for collagenous fibers, Gordon -Sweats's method for reticular fibers and Weigert's fucsine-resorcine method for elastic fibers. A large variability in quantity, distribution and characteristics of the matrix components was observed in the different types of neoplasms. Collagen type I, III and elements of the elastic system had different distribution in benign and malignant neoplasms. The simple Picrossirius method and under polarization enabled visualization of collagen as thick fibers irregularly distributed in the stroma of carcinomas and in a more orderly and regular fashion in benign neoplasms. A smaller amount of thin fibers was observed in an irregular and random disposition in carcinomas and in a regular disposition in benign neoplasms . Under polarization, the fibers present different lengths, were yellowish or reddish and strongly birefringent, what suggested that they were collagen type I and in the middle of these fibers, other ones, pale, greenish and weakly birefringent, some of them thinner, possibly collagen type III were observed. In the stroma of carcinoma, fibers were mostly thick, strongly birefringent, yellowish or reddish, disposed in an irregular and random fashion, mainly in the central areas. In condrometaplasic areas, both in malignant and benign neoplasms, there was a collagen population c |
| Disciplines: | Medicina veterinaria y zootecnia, Química, Biología |
| Keyword: | Pequeñas especies, Bioquímica, Biología celular, Diagnóstico, Neoplasias mamarias, Colágeno, Perros, Extracellular matrix, Matriz extracelular, Histología |
| Keyword: | Veterinary medicine and animal husbandry, Chemistry, Biology, Companion animals, Biochemistry, Cell biology, Diagnosis, Mammary neoplasms, Collagen, Dogs, Extracellular matrix, Histology |
| Full text: | Texto completo (Ver HTML) |
