Induction of chalcone synthase and phenylalanine ammonia-lyase by salicylic acid and Colletotrichum lindemuthianum in common bean
Document title: Induction of chalcone synthase and phenylalanine ammonia-lyase by salicylic acid and Colletotrichum lindemuthianum in common bean
Journal: Brazilian journal of plant physiology
Database: PERIÓDICA
System number: 000276113
ISSN: 1677-0420
Authors: 1
2
3



4
Institutions: 1Empresa Brasileira de Pesquisa Agropecuaria, Clima Temperado, Pelotas, Rio Grande do Sul. Brasil
2Universidade Federal do Rio Grande do Sul, Departamento de Botanica, Porto Alegre, Rio Grande do Sul. Brasil
3Universidade Federal do Rio Grande do Sul, Departamento de Botanica, Porto Alegre, Rio Grande do Sul. Brasil
4Universidade Federal de Pelotas, Instituto de Fisica e Matematica, Pelotas, Rio Grande do Sul. Brasil
Year:
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, analítico
English abstract The activities of the enzymes chalcone synthase (CHS) and phenylalanine ammonia-lyase (PAL) were measured in leaf extracts obtained from four cultivars of the common bean (AB 136, Rio Tibagi, Carioca and Macanudo). Two stages of plant development were examined: plantlets (V2) and the onset of blooming (R6). Initially, the plants were either treated with salicylic acid or inoculated with the delta race of Colletotrichum lindemuthianum (inductive fungus) and after three days they were evaluated for enzyme activity. Afterwards, all plants were inoculated (challenged) with the virulent pathotype 33/95 of C. lindemuthianum except for the water control. Five days later, the activities of PAL and CHS were evaluated. There were significant changes in the activities of both enzymes three days after treatment with salicylic acid or inductive fungus when compared to the control. Five days after inoculation with with the virulent pathotype 33/95 of C. lindemuthianum CHS activity in the Macanudo was similar to control plants that were not treated with salicylic acid or the inductive fungus but inoculated with 33/95 C. lindemuthianum. The increase in enzyme activity after challenge with 33/95 C. lindemuthianum was greatest for the salicylic acid treatment in the cultivar AB 136, followed by Rio Tibagi and Carioca
Portuguese abstract As atividades das enzimas chalcona sintase (CHS) e fenilalanina amônia-liase (PAL) foram avaliadas em extratos de folhas de quatro cultivares de feijão (AB 136, Rio Tibagi, Carioca e Macanudo), considerando-se dois estádios de desenvolvimento das plantas: plântulas (V2) e início de floração (R6). Inicialmente, as plantas foram tratadas com ácido salicílico ou inoculadas com a raça delta de Colletotrichum lindemuthianum (fungo indutor) e, após três dias, avaliadas quanto à atividade das enzimas. Em seguida, as plantas foram inoculadas com o patótipo virulento 33/95 de C. lindemuthianum, exceto as plantas-controle. Após cinco dias, avaliaram-se as atividades de PAL e CHS, observando-se acréscimos na atividade das enzimas, tanto no tratamento com ácido salicílico quanto com o fungo indutor, quando comparados com o controle. Houve alterações significativas nas atividades das enzimas depois de três dias de exposição aos tratamentos indutivos com ácido salicílico e fungo, quando comparados com o controle não induzido. Cinco dias após a inoculação com o patótipo virulento 33/95 de C. lindemuthianum, a atividade de CHS em Macanudo foi semelhante às plantas-controles que não haviam sido induzidas, mas inoculadas com 33/95 C. lindemuthianum. O aumento das atividades depois do desafio com 33/95 C. lindemuthianum foi maior nas plantas tratadas com ácido salicílico no cultivar AB 136, seguido por Rio Tibagi e Carioca
Disciplines: Biología,
Química,
Agrociencias
Keyword: Hongos,
Bioquímica,
Leguminosas,
Frijol,
Colletotrichum lindemuthianum,
Phaseolus vulgaris,
Resistencia,
Acido salicílico,
Enzimas
Keyword: Biology,
Chemistry,
Agricultural sciences,
Fungi,
Biochemistry,
Legumes,
Bean,
Phaseolus vulgaris,
Resistance,
Salicylic acid,
Enzymes,
Colletotrichum lindemuthianum
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