| Journal: | Brazilian Journal of Pharmaceutical Sciences |
| Database: | PERIÓDICA |
| System number: | 000451286 |
| ISSN: | 1984-8250 |
| Authors: | Taghiloo, Saeid1 Allahmoradi, Esmaeil1 Sadeghian Kiadehi, Seyedeh Forough1 Omrani Nava, Versa1 Nazar, Eisa2 Ebrahimzadeh, Mohammad Ali3 |
| Institutions: | 1Mazandaran University of Medical Sciences, School of Medicine, Sarí, Mazandarán. Irán 2Mashhad University of Medical Sciences, Department of Biostatistics and Epidemiology, Mashhad. Irán 3Mazandaran University of Medical Sciences, Hemoglobinopathy Institute , Sarí, Mazandarán. Irán |
| Year: | 2020 |
| Volumen: | 56 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Donkey’s milk represents a good alternative to human milk because of its chemical characteristics similar to those of human’s. In present study, the pro- and anti-inflammatory effects of donkey’s milk were evaluated on peripheral blood mononuclear cells (PBMCs). PBMCs were isolated from 12 young and 12 aged normal subjects. PBMCs were cultured with or without the optimal and non-cytotoxic dose of pasteurized donkey’s milk, and polymyxin B was used to inhibit the possible endotoxin contamination. Following 18 hours incubation, culture supernatants were harvested to measure the secreted Tumor necrosis factor-α (TNF-α), Interleukin-6 (IL-6), Interleukin-8 (IL-8) and Interleukin-10 (IL-10) by ELISA. Donkey’s milk significantly increased TNF-α (p= 0.01), IL-8 (p< 0.0001), IL-6 (p< 0.0001) and IL-10 (p= 0.01) levels in PBMCs. In addition, the levels of IL-6 (p= 0.002), IL-8 (p= 0.002) and TNF-α (p= 0.002) from aged subjects were significantly higher compared with young subjects. In contrast with these data, the level of IL-10 was markedly reduced from aged subjects (p= 0.02). Considering the immune-potentiation effects of donkey’s milk, it is suggested investigating milk as a beneficial dietary component for up-regulating the immune response in aged people |
| Disciplines: | Medicina |
| Keyword: | Inmunología, Sistema inmune, Citocinas, Leche de mono, ELISA |
| Keyword: | Immunology, *, Donkey milk, ELISA, Immune system, Cytokines |
| Full text: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
