| Journal: | Brazilian Journal of Pharmaceutical Sciences |
| Database: | PERIÓDICA |
| System number: | 000451950 |
| ISSN: | 1984-8250 |
| Authors: | Owjfard, Maryam1 Bigdeli, Mohammad Reza1 Safari, Anahid2 Namavar, Mohammad Reza3 |
| Institutions: | 1Shahid Beheshti University, Faculty of Biological Sciences, Teherán. Irán 2Shiraz University of Medical Sciences, Stem Cells Technology Research Center, Shiraz. Irán 3Shiraz University of Medical Sciences, Clinical Neurology Research Center, Shiraz. Irán |
| Year: | 2022 |
| Volumen: | 58 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Blood-brain barrier (BBB) disruption, inflammation, and cell death are major pathogenic mechanisms in ischemic stroke. Dimethyl fumarate (DMF) has anti-inflammatory and immune-modulatory effects. So, this study aimed to elucidate the effects of DMF on brain ischemia in the middle cerebral artery occlusion (MCAO) model. 69 Sprague-Dawley male rats were allocated into a sham group that was just subjected to surgery stress; vehicle and DMF groups, after MCAO, received vehicle or 30 mg/kg DMF for three days. Neurological scores were evaluated every day. BBB disruption was evaluated by the extravasation of Evans blue. In addition to the measurement of brain water content, the total and infarct volume, numerical density, and the total number of neurons, non-neurons, and dead neurons in the right cortex were estimated by stereological methods. RT-PCR was done to analyze the expression levels of NF-κB and Nrf2. Although brain ischemia treatment with DMF did not have a significant effect on the infarction size, it improved neurobehavioral function, BBB disruption, cerebral edema, increased number of neurons, and expression of Nrf2. It also decreased the number of dead neurons and the expression of NF-κB. DMF beneficial effects on stroke may be mediated through both increase of the Nrf2 and decrease of NF-κB expression |
| Disciplines: | Medicina |
| Keyword: | Neurología, Farmacología, Medicina experimental, Dimetil fumarato, Isquemia cerebral, Edema cerebral, Muerte celular, Modelos animales, Ratas |
| Keyword: | Neurology, Pharmacology, Experimental medicine, Dimethyl fumarate, Brain ischemia, Brain edema, Cell death, Animal models, Rats |
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