| Journal: | Brazilian Journal of Pharmaceutical Sciences |
| Database: | PERIÓDICA |
| System number: | 000451515 |
| ISSN: | 1984-8250 |
| Authors: | Brauer, Veronica Soares1 Zambuzi, Fabiana Albani1 Espíndola, Milena Sobral1 Cavalcanti, Marinaldo Pacífico1 Prado, Morgana Kelly Borges1 Cardoso, Priscilla Mariane1 Soares, Luana Silva1 Galvao Lima, Leonardo Judson1 Leopoldino, Andréia Machado1 Cardoso, Cristina Ribeiro de Barros1 Frantz, Fabiani Gai1 |
| Institutions: | 1Universidade de Sao Paulo, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Ribeirao Preto, Sao Paulo. Brasil |
| Year: | 2021 |
| Volumen: | 57 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Dehydroepiandrosterone (DHEA) is a steroid hormone secreted by the adrenal glands, gonads and brain. It is a precursor to sex hormones and also is known to have immune modulatory activity. However, little is known about the relationship between DHEA and neutrophils and thus our study evaluates the influence of DHEA in the effector functions of neutrophils. Human neutrophils were treated in vitro with DHEA and further infected with Salmonella enterica serovar Typhimurium. The treatment of neutrophils with 0.01 μM of DHEA increased the phagocytosis of Salmonella independent of TLR4 as the treatment did not modulate the TLR4 expression. Additionally, DHEA caused a decrease in ROS (reactive oxygen species) production and did not influence the formation of the neutrophil extracellular trap (NET). Steroid treated neutrophils, infected or stimulated with LPS (lipopolysaccharide), showed reduced production of IL-8, compared to untreated cells. Also, the protein levels of p-NFκB were decreased in neutrophils treated with DHEA, and this reduction could explain the reduced levels of IL-8. These results led us to conclude that the steroid hormone DHEA has important modulatory functions in neutrophils |
| Disciplines: | Medicina |
| Keyword: | Bioquímica, Neuroinmunomodulación, Dehidroepiandrosterona, Neutrófilos, Inmunidad innata, Infección experimental, Salmonella typhimurium, Fagocitosis |
| Keyword: | Biochemistry, Neuroimmunomodulation, Dehydroepiandrosterone, Neutrophils, Innate immunity, Experimental infection, Salmonella typhimurium, Phagocytosis |
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