| Journal: | Brazilian Journal of Pharmaceutical Sciences |
| Database: | PERIÓDICA |
| System number: | 000451935 |
| ISSN: | 1984-8250 |
| Authors: | Hosseini, Seyed Mohammad Karim1 Alizadeh, Fahimeh1 Nouripour Sisakht, Sadegh2 Khodavandi, Alireza3 |
| Institutions: | 1Islamic Azad University, Yasouj Branch, Yasouj. Irán 2Yasuj University of Medical Sciences, Cellular and Molecular Research Center, Yasouj. Irán 3Islamic Azad University, Gachsaran Branch, Gachsaran. Irán |
| Year: | 2022 |
| Volumen: | 58 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Candida glabrata infections are responsible for deaths of people globally. Fluconazole is known to be less effective against C. glabrata, which developed many strategies to evade being destroyed by fluconazole. To achieve enhanced efficacy of fluconazole against C. glabrata, the interaction of fluconazole with sodium bicarbonate was investigated using the CLSI guidelines. The efficacy of fluconazole alone and in combination with sodium bicarbonate was evaluated using the time-kill and phospholipase production assays. Eventually, the expression of PLB was assessed using semi-quantitative RT-PCR to investigate the inhibitory properties of fluconazole alone and in combination with sodium bicarbonate against C. glabrata. The fluconazole/sodium bicarbonate combination displayed synergistic and antagonistic effects (FICI= 0.375-4.25). In C. glabrata ATCC, SN 152, and SN 164, the fluconazole/sodium bicarbonate combination exhibited a significant fungicidal activity (p< 0.05) but antagonistic effect in the case of SN 283. With exception of SN 283, a significant reduction was noted in phospholipase production in clinical isolates of C. glabrata treated with fluconazole/sodium bicarbonate combination. The PLB was down-regulated significantly by 0.168-0.515 fold in C. glabrata treated with fluconazole/sodium bicarbonate. The results suggested fluconazole/sodium bicarbonate to have a potential synergistic interaction in C. glabrata, and the underlying mechanism may be associated with phospholipase gene |
| Disciplines: | Química, Biología |
| Keyword: | Farmacología, Fluconazol, Bicarbonato de sódio, Sinergismo, Candida glabrata |
| Keyword: | Pharmacology, Fluconazole, Sodium bicarbonate, Synergism, Candida glabrata |
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