| Journal: | Brazilian Journal of Pharmaceutical Sciences |
| Database: | PERIÓDICA |
| System number: | 000451242 |
| ISSN: | 1984-8250 |
| Authors: | Vijayaraj, Surendran1 Palei, Narahari N2 Archana, Devalapalli1 Lathasri, Kuppam2 Rajavel, Ponnusamy1 |
| Institutions: | 1Sree Vidyanikethan College of Pharmacy, Department of Pharmaceutical Analysis, Tirupati, Andhra Pradesh. India 2Sree Vidyanikethan College of Pharmacy, Department of Pharmaceutics, Tirupati, Andhra Pradesh. India |
| Year: | 2020 |
| Volumen: | 56 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Rutin is a flavonoid glycoside, mainly consists of phenolic compounds, responsible for many biological activities. The objective of the present study was to develop and validate a precise, simple, robust, rapid and reliable reverse phase high -performance liquid chromatography (RP-HPLC) technique by using Qbd approach for evaluating the rutin in nanoparticles. Central composite design (CCD) was employed for optimizing the experimental conditions of RP-HPLC method. Buffer pH, methanol content in the mobile phase composition, flow rate, and wavelength were selected as independent variables whereas retention time, peak area, and asymmetry factor was selected as dependent variables. The retention time, peak area and asymmetric factor of rutin by using optimized independent variables were found to be 3.75 min, 1014.79 mV, and 1.26 respectively. The limit of detection and limit of quantitation values were found to be 0.005 µg/mL and 0.15 µg/mL respectively. For confirming linearity, accuracy, precision, and robustness, the optimized assay condition was validated as per ICH guidelines. The proposed method, which was optimized by QbD approach was found to be a suitable method for analyzing the rutin in chitosan-sodium alginate nanoparticles |
| Disciplines: | Química |
| Keyword: | Química farmacéutica, Química analítica, Rutina, Nanopartículas, Quitosano, Alginato de sodio, Cromatografía líquida de alta resolución (HPLC), Diseño central compuesto |
| Keyword: | Medicinal chemistry, Analytical chemistry, Rutin, Chitosan, Sodium alginate, Nanoparticles, High performance liquid chromatography (HPLC), Central composite design |
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