Preparation and in vitro release profiling of PLGA microspheres containing BSA as a model protein
Document title: Preparation and in vitro release profiling of PLGA microspheres containing BSA as a model protein
Journal: Brazilian Journal of Pharmaceutical Sciences
Database: PERIÓDICA
System number: 000451225
ISSN: 1984-8250
Authors: 1
2
1
3
Institutions: 1Tehran University of Medical Sciences, School of Pharmacy, Teherán. Irán
2University of Auckland, Department of Molecular Medicine and Pathology, Auckland, Central Auckland. Nueva Zelanda
3Alborz University of Medical Sciences, Evidence-based Phytotherapy & Complementary Medicine Research Center, Karaj. Irán
Year:
Volumen: 56
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract Conventional drug formulations are incapable of adequate delivery of proteins and peptides for therapeutic purposes. As these molecules have very short biological half-life, multiple dosing is required to achieve the desirable therapeutic effects. Microspheres are able to encapsulate proteins and peptide in the polymeric matrix while protecting them from enzymatic degradation. In this study Bovine Serum Albumin (BSA) matrix type microspheres were fabricated using Polylactide-co-glycolide (PLGA) by double emulsion solvent evaporation method. The effects of variables such as homogenizer speed, molecular weight of polymer and the effect of pH of the water phases, were investigated against factors such as drug loading, encapsulation efficiency, morphology, size, drug distribution and release profile of the microspheres. Results, suggested that an increase in homogenization speed leads to a decrease in microsphere size. The increase in homogenization speed also caused a significant effect on the release profile only when higher molecular weight of polymer had been used.. The pH change of the internal aqueous phase led to modification of surface morphology of spheres to a porous structure that significantly increased the total amount of released protein. Integrity of protein structure was intact as shown by SDS-PAGE. According to the results, it can be concluded that we achieved a reproducible method regarding controlled protein delivery for different sizes of particles
Disciplines: Química
Keyword: Química farmacéutica,
Liberación de fármacos,
Proteínas,
Encapsulación,
Microesferas,
Acido poliláctico-co-glicólico,
Perfil de liberación
Keyword: Medicinal chemistry,
Drug delivery,
Proteins,
Encapsulation,
Microspheres,
Polylactic-co-glycolic acid,
Release profile
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