| Journal: | Brazilian Journal of Pharmaceutical Sciences |
| Database: | PERIÓDICA |
| System number: | 000451755 |
| ISSN: | 1984-8250 |
| Authors: | Hinge, Madhuri Ajay1 Patel, Dipti2 |
| Institutions: | 1ROFEL Shri G.M. Bilakhia College of Pharmacy, Department of Pharmaceutical Quality Assurance, Vapi, Gujarat. India 2Ganpat University, SHREE S.K. PATEL College of Pharmaceutical Education and Research, Mehsana, Gujarat. India |
| Year: | 2022 |
| Volumen: | 58 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | A simple, precise, accurate and robust high performance liquid chromatographic method has been developed for simultaneous estimation of Torsemide and Eplerenone in tablet dosage form. Design of experiment was applied for multivariate optimization of the experimental conditions of RP-HPLC method. A Central composite design was used to study the response surface methodology and to analyse in detail the effects of these independent factors on responses. Total eleven experiments along with 3 center points were performed. Two factors were selected to design the matrix, one factor is variation in ratio of Acetonitrile and the second factor is flow rate (mL/min). Optimization in chromatographic conditions was achieved by applying Central composite design. The optimized and predicted data from contour diagram comprised mobile phase (acetonitrile, water and methanol in the ratio of 50: 30: 20 v/v/v respectively), at a flow rate of 1.0 ml/min and at ambient column temperature. Using these optimum conditions baseline separation of both drugs with good resolution and run time of less than 5 minutes were achieved. The optimized assay conditions were validated as per the ICH guidelines (2005). Hence, the results showed that the Quality by design approach could successfully optimize RP-HPLC method for simultaneous estimation of Torsemide and Eplerenone |
| Disciplines: | Química |
| Keyword: | Química farmacéutica, Química analítica, Tabletas, Torsemida, Eplerenona, Cromatografía líquida de alta resolución (HPLC) |
| Keyword: | Medicinal chemistry, Analytical chemistry, Tablets, Torsemide, Eplerenone, High performance liquid chromatography (HPLC) |
| Full text: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
