| Journal: | Brazilian Journal of Pharmaceutical Sciences |
| Database: | PERIÓDICA |
| System number: | 000451426 |
| ISSN: | 1984-8250 |
| Authors: | Thongchai, Wisanu1 |
| Institutions: | 1Pibulsongkram Rajabhat University, Faculty of Science and Technology, Phitsanulok. Tailandia |
| Year: | 2020 |
| Volumen: | 56 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Ion-pairing liquid chromatographic method was validated for determination of ketoconazole in shampoo and cream samples as per ICH guidelines. The chromatographic conditions were carried out in the isocratic mode using a mixture of methanol and 8 mM sodium dodecyl sulfate (pH 5.5) in a ratio of 45:55 v/v %, as mobile phase. The flow rate was set at 1.0 mL min-1. Chromolith RP-18e (100×4.6 mm) was used as the analytical column with a fluorescence detection at an excitation wavelength of 260 nm and an emission wavelength of 375 nm. The average percentage recovery of shampoo A, shampoo B, shampoo C, cream A and cream B were 99.88, 97.06, 99.58, 96.77 and 97.26, respectively. The limit of detection was 0.12 mg L-1. The drug decomposition under acid degradation, base degradation and oxidative degradation were found to be in the range of 91.63-94.70% indicating that the drug is resistant towards acidic conditions. The drug decomposition under thermal condition and photolysis condition were found to be in the range of 69.05-87.15% and 47.31-66.83% respectively, indicating that the drug decomposition is more sensitive under photolysis conditions. This method is suitable for the quality control of ketoconazole in commercial shampoo and creams |
| Disciplines: | Química |
| Keyword: | Química farmacéutica, Química analítica, Formulaciones farmacéuticas, Champú, Cremas, Ketoconazol, Detección por fluorescencia, Cromatografía líquida por acoplamiento iónico |
| Keyword: | Medicinal chemistry, Analytical chemistry, Pharmaceutical formulations, Ion-pairing liquid chromatography, Ketoconazole, Shampoo, Creams, Fluorescence detection |
| Full text: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
