| Journal: | Brazilian Journal of Pharmaceutical Sciences |
| Database: | PERIÓDICA |
| System number: | 000451437 |
| ISSN: | 1984-8250 |
| Authors: | Campos, Tarcisio Passos Ribeiro de1 Dalmazio, Ilza2 Augusti, Rodiney3 Almeida, Iassudara Garcia1 |
| Institutions: | 1Universidade Federal de Minas Gerais, Departamento de Engenharia Nuclear, Belo Horizonte, Minas Gerais. Brasil 2Universidade Federal de Minas Gerais, Centro de Desenvolvimento da Tecnologia Nuclear, Belo Horizonte, Minas Gerais. Brasil 3Universidade Federal de Minas Gerais, Instituto de Quimica, Belo Horizonte, Minas Gerais. Brasil |
| Year: | 2020 |
| Volumen: | 56 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Breast cancer cell uptake of Gd-metal is investigated based on the formation of coordinate compounds of gadolinium and glucose (Glu) molecules in solution. The hypothesis is that glucose helps Gd-internalization by complex formations constituted of Gd3+ coordinate to m-glucose molecules, whose valence was complemented by Cl- anions. Such a proposal is an insight toward a metabolic-dependent contrast-agent for cancer and inflammation in magnetic resonance image. A solution was prepared based on anhydrous d-glucose and gadolinium chloride (Gd-Glu). Uptake assays for MDA-MB-231(c231) cells were elaborated collecting incubated c231-cells with Gd-Glu and measuring metal-uptake and their concentrations by Nuclear Activation Analysis (NAA). The ionic solution was studied using Direct-Infusion Electrospray Ionization Mass-Spectrometry (ESI-MS) to identify Gd-Glu interactions. Means and standard deviations of Gd-masses were 13.3±0.8 and 12.5±0.7µg, at 361.5 µg of Gd in 3mL Gd-Glu/PBS solution, in times of 30-50 min, equivalent to the concentrations of 13404±2104 and 11347±2742 µg.g-1 in dried cells. Such values were statistically higher than the control with metal presence. ESI-MS demonstrated the m/z-signals at 516, 552, 696, 923, attributed to positively loaded-species containing Glu, Gd+3 and Cl-. In conclusion, Gd-internalization was increased in aqueous solution due to the gadolinium-glucose coordination. Such findings drive the research to MRI with Gd-Glu complexes |
| Disciplines: | Química, Medicina |
| Keyword: | Oncología, Farmacología, Medicina experimental, Cáncer, Mama, Compuestos de coordinación, Gadolinio, Glucosa, Ensayo de Absorción celular, Oncology |
| Keyword: | Pharmacology, Experimental medicine, Cancer, Breast, Coordination compounds, Glucose, Gadolinium, Cell uptake assay |
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