| Journal: | Brazilian Journal of Pharmaceutical Sciences |
| Database: | PERIÓDICA |
| System number: | 000451233 |
| ISSN: | 1984-8250 |
| Authors: | Koga, Luciana Numata1 Fenelon, Vanderson Carvalho1 Miyoshi, Juliana Harumi1 Moriwaki, Cristiane1 Wessel, Kamila Byanca Baldin2 Mangolim, Camila Sampaio3 Matioli, Graciette1 |
| Institutions: | 1Universidade Estadual de Maringa, Departamento de Farmacia, Maringa, Parana. Brasil 2Universidade Estadual de Londrina, Londrina, Parana. Brasil 3Universidade Federal do Paraiba, Departamento de Gestao e Tecnologia Industrial, Joao Pessoa, Paraiba. Brasil |
| Year: | 2020 |
| Volumen: | 56 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Aplicado, descriptivo |
| English abstract | A repetitive batch process was employed followed by membrane ultrafiltration system to produce low-cost cyclodextrins (CDs) using commercial enzymes Toruzyme® cyclomaltodextrin glucanotransferase (CGTase) and its kinetic parameters were determined. The ultrafiltration system enabled the removalof inhibitory products from the reaction medium, allowing the enzyme to be recovered for reuse. A 10 kDa membrane was used to separate the different CDs produced by the CGTase. The substrates evaluated were maltodextrin, corn starch and cassava starch at 5, 10 and 15% (w/V), in the presence and absence of 10% (V/V) ethanol. After reaction for 132 h, 10% (w/V) cassava starch in the presence of ethanol provided the best results with 32.1 mg/mL of β-CD. Maximum production occurred after 72 h of reaction, with a yield of 87.4% of β-CD and an α-CD, β-CD and γ-CD production ratio of 1:1:0.08 g, respectively. When eight repetitive batches of 72 h followed by ultrafiltration and crystallization of β-CD were performed, 2.1 g of precipitate was obtained with a purity of 67.6% β-CD. The supernatant from the crystallization process was lyophilized and resulted in 35.3% α-CD. The developed model can be used industrially for the production of low cost CDs from easily obtained raw material |
| Disciplines: | Química |
| Keyword: | Química farmacéutica, Química industrial, Ciclodextrina, Producción industrial, Toruzyme, Ultrafiltración |
| Keyword: | Medicinal chemistry, Industrial chemistry, Cyclodextrin, Industrial production, Toruzyme, Ultrafiltration |
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