Development and validation of highly selective method for the determination of imatinib mesylate and dexketoprofen trometamol combination in three different media
Document title: Development and validation of highly selective method for the determination of imatinib mesylate and dexketoprofen trometamol combination in three different media
Journal: Brazilian Journal of Pharmaceutical Sciences
Database: PERIÓDICA
System number: 000451334
ISSN: 1984-8250
Authors: 1
2
Institutions: 1Karadeniz Technical University, Faculty of Pharmacy, Trabzon. Turquía
2Biruni University, Faculty of Pharmacy, Estambul. Turquía
Year:
Volumen: 56
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract Imatinib mesylate is a small molecule used in cancer therapy as a thyrosine kinase inhibitor. Dexketoprofen trometamol is a non-steroidal anti-inflammatory drug that has seen use in cancer therapy in combination with an anticancer drug to minimize tumor size and to reduce pain in patients. In the present study, imatinib mesylate and dexketoprofen trometamol were selected as potential model drugs to be used in combination. A new, simple and selective Ultra Performance Liquid Chromatography method was developed and validated to determine the drug substances in distilled water, in a pH 7.4 phosphate buffer and in Dulbecco’s Modified Eagle Medium. The proposed method was developed using a BEH C-18 column with isocratic elution. A mixture of methanol:acetonitrile (80:20, v/v) and pH 9.5, 0.05 M ammonium acetate were (70:30, v/v) used as a mobile phase. Detection was carried out with a flow rate of 0.3 mL/min, a column temperature of 30°C and an injection volume of 20 μL. The method was validated considering linearity, accuracy, precision, specificity, robustness, detection limit and quantitation limit values, and was found to be linear in a range from 0.05 to 20.0 μg/mL for the three different media
Disciplines: Química
Keyword: Química farmacéutica,
Química analítica,
Mesilato de imatinib,
Trometamol dexketoprofeno,
Métodos analíticos,
Cromatografía líquida de alta resolución (HPLC)
Keyword: Medicinal chemistry,
Analytical chemistry,
Analytical methods,
Imatinib mesylate,
Dexketoprofen trometamol,
High performance liquid chromatography (HPLC)
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