| Journal: | Brazilian Journal of Pharmaceutical Sciences |
| Database: | PERIÓDICA |
| System number: | 000451134 |
| ISSN: | 1984-8250 |
| Authors: | Wei, Dai1 Yun, LI Shi1 Dejun, Xiao1 Cong, Liu1 He, Jin-Hua2 Yan, Lin1 |
| Institutions: | 1Gangzhou People’s Hospital, Department of Clinical Laboratory, Ganzhou, Jiangxi. China 2Central Hospital of Panyu District, Department of Laboratory Medicine, Guangzhou, Guangdong. China |
| Year: | 2019 |
| Volumen: | 55 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | To study the effect of small interfering RNA targeting metastasis-associated lung adenocarcinoma transcript1 (si-MALAT1) combining with curcumin on the invasion and migration abilities of human colon cancer SW480 cells, and to explore the involved molecular mechanism. The recombinant lentiviral vector expressing si-MALAT1 was constructed, and its titer was determined by gradient dilution method. The colon cancer SW480 cells with stable expression of si-MALAT1 was established, followed by treatment with curcumin at different concentrations. The effect of curcumin or si-MALAT1 alone and the combination of the two on the cell activity was detected by MTT assay. The cell invasion and migration abilities were detected by transwell and scratch-wound assay. The relative expression level of MALAT1 was detected by RT-qPCR. The protein expression was determined by Western blot analysis. The IC50 of curcumin alone was 77.69 mmol/L, which was 51.17 mol/L when combined with curcumin and random sequence. The IC50 of curcumin was 30.02 mmol/L when combined with si-MALAT1. The increased susceptibility multiples was 2.58. The wound healing rates were 30.9% and 67.5% after treatment with si-MALAT1 combined with curcumin for 24 hrs and 48 hrs, respectively. The numbers of invasion cells were 200±12, 162±13, 66±8, 53±4 and 16±3 after treatment with si-MALAT1 combined with curcumin for 48 hrs. The relative expression level of lncRNA-MALAT1 in the curcumin group was 68%, and the relative expression level of lncRNA-MALAT1 in si-MALAT1group was 56%, and that for the combination treatment group was about 21%. The protein expression levels of β- catenin, c-myc and cyclinD1 were significantly down-regulated upon treatment with certain concentration of si-MALAT1 alone or combined with curcumin.si-MALAT1 could significantly inhibit the invasion and migration of SW480 cells by enhancing the sensitivity of SW480 cells to curcumin. The mechanism involved mignt be related to the do |
| Disciplines: | Medicina, Química |
| Keyword: | Oncología, Farmacología, ARN no codificante, Cáncer colorrectal, Inhibición, Migración celular, Curcumina |
| Keyword: | Oncology, Pharmacology, Non-coding RNA, Colorectal cancer, Inhibition, Cell migration, Curcumin |
| Full text: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
