Anti-Acanthamoeba castellanii activity of alkaloid-enriched extracts and lycorine from the Amaryllidaceae species
Document title: Anti-Acanthamoeba castellanii activity of alkaloid-enriched extracts and lycorine from the Amaryllidaceae species
Journal: Brazilian Journal of Pharmaceutical Sciences
Database: PERIÓDICA
System number: 000451711
ISSN: 1984-8250
Authors: 1
2
3
2
4
2
1
Institutions: 1Universidade Federal do Espirito Santo, Centro de Ciencias da Saude, Vitoria, Espirito Santo. Brasil
2Universidade Federal do Espirito Santo, Departamento de Quimica, Vitoria, Espirito Santo. Brasil
3Universidade Federal de Minas Gerais, Faculdade de Farmacia, Belo Horizonte, Minas Gerais. Brasil
4Universidad de Barcelona, Facultad de Farmacia, Barcelona. España
Year:
Volumen: 58
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract Free-living amoebae of the genus Acanthamoeba are the causative agents of granulomatous encephalitis and keratitis, severe human infections. Bioactive compounds from plants are recognized as an alternative source for the development of new drugs. The Amaryllidaceae is a botanical family able to synthesize a very specific and consistent group of biologically active isoquinoline-like alkaloids. The alkaloidal fractions from the Brazilian species Hippeastrum canastrense, H. diniz-cruziae, H. puniceum, and Crinum x amabile, along with the alkaloid lycorine, were investigated against Acanthamoeba castellanii. The in vitro assays were performed with distinct concentrations of lycorine and alkaloidal fractions, while the cell viability was evaluated by the MTT method upon MDCK cells. Chlorhexidine 0.02% was used as the positive control. The effect of alkaloid fractions was concentration dependent, and 2000 μg mL-1 of H. canastrense and H. diniz-cruziae provided a 100% inhibition. At concentrations of 250, 500, and 1000 μg mL-1, the H. diniz-cruziae alkaloidal fraction showed the lowest cytotoxic effect (5%-7%) and remarkable anti-amoebic activity, demonstrating values of IC50 285.61 μg mL-1, low cytotoxicity (5%-7%), and selectivity index (7.0). Taken together, the results are indicative of the great potential that the alkaloids from H. diniz-cruziae have as new candidates for anti-amoebicidal compounds
Disciplines: Biología,
Química
Keyword: Fitoquímica,
Microbiología,
Extractos vegetales,
Alcaloides,
Citotoxicidad,
Acanthamoeba castellanii,
Hippeastrum canastrense,
Hippeastrum diniz-cruziae,
Hippeastrum puniceum,
Amaryllidaceae
Keyword: Phytochemistry,
Microbiology,
Plant extracts,
Alkaloids,
Cytotoxicity,
Acanthamoeba castellanii,
Hippeastrum canastrense,
Hippeastrum diniz-cruziae,
Hippeastrum puniceum,
Amaryllidaceae
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