| Journal: | Brazilian Journal of Pharmaceutical Sciences |
| Database: | PERIÓDICA |
| System number: | 000451519 |
| ISSN: | 1984-8250 |
| Authors: | Fatma, Zioud1 Luis, Martín Barrasa Jose1 Antonio, Espinosa De Los Monteros Y Zaya2 Laura, Santana Reyes2 Pherraez, Herráez Thomas2 María, González Martín Jesús1 Ángela, Ramos Nuez3 Rafik, Bachoual4 |
| Institutions: | 1Hospital Universitario Dr. Negrín, Unidad de Investigación, Las Palmas de Gran Canaria. España 2Universidad de Las Palmas de Gran Canaria, Instituto Universitario de Sanidad Animal y Seguridad Alimentaria, Arucas, Las Palmas de Gran Canaria. España 3Hospital Universitario Dr. Negrín, Red Multidisciplinaria de Investigación y Evaluación de disfunción Orgánica, Las Palmas de Gran Canaria. España 4University of Gabes, Faculty of Sciences of Gabes, Gabes. Túnez |
| Year: | 2021 |
| Volumen: | 57 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | We investigated the effect of Punica granatum peel aqueous extract (PGE), on pulmonary inflammation and alveolar degradation induced by intratracheal administration of Elastase in Sprague Dawley rats. Lung inflammation was induced in rats by intratracheal instillation of Elastase. On day 1 and 2, animals received an intraperitoneal injection of PGE (200 mg/mL), three hours later, they were intratracheally instilled with 25U/kg pancreatic porcine Elastase. Animals were sacrificed 7 days later. Bronchoalveolar lavage (BAL) were collected and cellularity, histology and mRNA expression of Monocyte chemotactic protein 1(MCP-1), Tumor Necrosis Factor-Alpha (TNF-α), Interleukin 6 (IL-6), and Matrix Metalloproteinase-2 (MMP-2) were studied. In addition, activity of TNF- α, IL-6 and MCP-1 on BAL were also analyzed by ELISA Kit. Elastase administration increased: BAL cellularity, neutrophils recruitment and BAL MCP1, IL-6 expressions. It also increased lung TNF-α, MCP-1, MMP-2 expressions, platelets recruitment, histological parameters at 7th day of elastase treatment. Intraperitoneal injection of 200 mg/kg of PGE reduced, significantly, BAL cellularity, and neutrophils recruitment. However, in animal treated with PGE, MCP-1, MMP-2 and IL-6 on day 7, were similar to the Sham group. Treatment with PGE (200 mg/ kg) also significantly reduced lung TNF-α, and MCP-1 expression. This study reveals that PGE Punica granatum protects against elastase lung inflammation and alveolar degradation induced in rats |
| Disciplines: | Química, Medicina |
| Keyword: | Farmacología, Medicina experimental, Edema pulmonar, Elastasa, Enfisema, Extractos vegetales, Punica granatum, Punicaceae, Ratas |
| Keyword: | Pharmacology, Experimental medicine, Pulmonary edema, Elastase, Emphysema, Plant extracts, Punica granatum, Punicaceae, Rats |
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