| Journal: | Brazilian journal of microbiology |
| Database: | PERIÓDICA |
| System number: | 000291252 |
| ISSN: | 1517-8382 |
| Authors: | Rosalem-Junior, Wilson1 Souza, Rodrigo Carvalho de2 Andrade, Arnaldo Feitosa Braga de Colombo, Ana Paula Vieira3 |
| Institutions: | 1Universidade do Estado do Rio de Janeiro, Departamento de Microbiologia, Rio de Janeiro. Brasil 2Universidade Federal do Rio de Janeiro, Departamento de Periodontologia, Rio de Janeiro. Brasil 3Universidade Federal do Rio de Janeiro, Instituto de Microbiologia, Rio de Janeiro. Brasil |
| Year: | 2006 |
| Season: | Abr-Jun |
| Volumen: | 37 |
| Number: | 2 |
| Pages: | 127-134 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Caso clínico |
| English abstract | Actinobacillus actinomycetemcomitans (Aa) is considered a major etiologic agent of aggressive periodontitis but this species has also been associated with other forms of periodontal disease. Further, highly leukotoxic strains are related to severity of disease. The purpose of this study was to determine the prevalence of Aa and the occurrence of the leukotoxin gene 530-bp deletion in patients with generalized aggressive periodontitis (GAP) from a Brazilian population. Thirty periodontally healthy and 29 GAP subjects participated in the study. Full-mouth periodontal examination including probing pocket depth (PPD), clinical attachment level (CAL), supragingival biofilm (SB) and bleeding on probing (BOP) was carried out at 6 sites/tooth for all subjects. Whole saliva samples were collected for bacterial DNA isolation. The detection of Aa and the presence of the 530-bp genetic deletion were determined directly in the samples by polimerase chain reaction. Differences on clinical and microbiological parameters between the two groups were sought using the Mann-Whitney, Fisher´s exact and Chi-square tests. Associations between clinical and microbiological parameters were tested using the Pearson correlation coefficient. Aa was detected significantly more often in GAP patients (96.6%) than healthy subjects (76.7%) (chi2 = 4.9; p < 0.05). The genetic deletion was observed in 16 of the 28 (57.1%) GAP patients who were positive for Aa. However, none of the samples from individuals with periodontal health presented this deletion (chi2 = 19 |
| Disciplines: | Medicina, Biología, Química |
| Keyword: | Microbiología, Odontología, Bacterias, Bioquímica, Periodontitis, Leukotoxina, PCR, Actinobacillus actinomycetemcomitans, Saliva |
| Keyword: | Medicine, Biology, Chemistry, Dentistry, Microbiology, Bacteria, Biochemistry, Periodontitis, Leukotoxin, PCR, Actinobacillus actinomycetemcomitans, Saliva |
| Full text: | Texto completo (Ver HTML) |
