| Journal: | Brazilian journal of medical and biological research |
| Database: | PERIÓDICA |
| System number: | 000349895 |
| ISSN: | 0100-879X |
| Authors: | Espirito-Santo, M.P1 Carneiro, M.A.S2 Reis, N.R.S2 Kozlowski, A.G2 Teles, S.A3 Lampe, E1 Yoshida, C.F.T1 Martins, R.M.B2 |
| Institutions: | 1Instituto Oswaldo Cruz, Departamento de Virologia, Rio de Janeiro. Brasil 2Universidade Federal de Goias, Instituto de Patologia Tropical e Saude Publica, Goiania, Goias. Brasil 3Universidade Federal de Goias, Faculdade de Enfermagem, Goiania, Goias. Brasil |
| Year: | 2007 |
| Season: | Abr |
| Volumen: | 40 |
| Number: | 4 |
| Pages: | 545-550 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | The present study examined the distribution of hepatitis C virus (HCV) genotypes and subtypes in a hemodialysis population in Goiás State, Central Brazil, and evaluated the efficiency of two genotyping methods: line probe assay (LiPA) based on the 5' noncoding region and nucleotide sequencing of the nonstructural 5B (NS5B) region of the genome. A total of 1095 sera were tested for HCV RNA by RT-nested PCR of the 5' noncoding region. The LiPA assay was able to genotype all 131 HCV RNA-positive samples. Genotypes 1 (92.4%) and 3 (7.6%) were found. Subtype 1a (65.7%) was the most prevalent, followed by subtypes 1b (26.7%) and 3a (7.6%). Direct nucleotide sequencing of 340 bp from the NS5B region was performed in 106 samples. The phylogenetic tree showed that 98 sequences (92.4%) were classified as genotype 1, subtypes 1a (72.6%) and 1b (19.8%), and 8 sequences (7.6%) as subtype 3a. The two genotyping methods gave concordant results within HCV genotypes and subtypes in 100 and 96.2% of cases, respectively. Only four samples presented discrepant results, with LiPA not distinguishing subtypes 1a and 1b. Therefore, HCV genotype 1 (subtype 1a) is predominant in hemodialysis patients in Central Brazil. By using sequence analysis of the NS5B region as a reference standard method for HCV genotyping, we found that LiPA was efficient at the genotype level, although some discrepant results were observed at the subtype level (sensitivity of 96.1% for subtype 1a and 95.2% for subtype 1b). Thus, analysis of the NS5B region permitted better discrimination between HCV subtypes, as required in epidemiological investigations |
| Disciplines: | Medicina |
| Keyword: | Gastroenterología, Microbiología, Epidemiología, Virus de la hepatitis C, Genotipificación, Hemodiálisis |
| Keyword: | Medicine, Gastroenterology, Microbiology, Epidemiology, Hepatitis C virus, Genotyping, Hemodialysis |
| Full text: | Texto completo (Ver HTML) |
