Effect of metabolic control on interferon-g and interleukin-10 production by peripheral blood mononuclear cells from type 1 and type 2 diabetic patients
Document title: Effect of metabolic control on interferon-g and interleukin-10 production by peripheral blood mononuclear cells from type 1 and type 2 diabetic patients
Journal: Brazilian journal of medical and biological research
Database: PERIÓDICA
System number: 000350343
ISSN: 0100-879X
Authors: 1
1
1
1
Institutions: 1Universidade de Sao Paulo, Faculdade de Medicina de Ribeirao Preto, Ribeirao Preto, Sao Paulo. Brasil
Year:
Season: May
Volumen: 40
Number: 5
Pages: 671-677
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract The objective of the present study was to evaluate the production of cytokines, interferon-g (INF-g) and interleukin-10 (IL-10), in cultures of peripheral blood mononuclear cells (PBMC) from type 1 and type 2 diabetic patients and to correlate it with inadequate and adequate metabolic control. We studied 11 type 1 and 13 type 2 diabetic patients and 21 healthy individuals divided into two groups (N = 11 and 10) paired by sex and age with type 1 and type 2 diabetic patients. The PBMC cultures were stimulated with concanavalin-A to measure INF-g and IL-10 supernatant concentration by ELISA. For patients with inadequate metabolic control, the cultures were performed on the first day of hospitalization and again after intensive treatment to achieve adequate control. INF-g levels in the supernatants of type 1 diabetic patient cultures were higher compared to type 2 diabetic patients with adequate metabolic control (P < 0.001). Additionally, INF-g and IL-10 tended to increase the liberation of PBMC from type 1 and 2 diabetic patients with adequate metabolic control (P = 0.009 and 0.09, respectively). The increased levels of INF-g and IL-10 released from PBMC of type 1 and 2 diabetic patients with adequate metabolic control suggest that diabetic control improves the capacity of activation and maintenance of the immune response, reducing the susceptibility to infections
Disciplines: Medicina
Keyword: Farmacología,
Metabolismo,
Diabetes,
Interleucina 10,
Interferón gama,
Células mononucleares,
Control metabólico
Keyword: Medicine,
Pharmacology,
Metabolism,
Diabetes,
Interleukin 10,
Gamma interferon,
Mononuclear cells,
Metabolic control
Full text: Texto completo (Ver HTML)