| Journal: | Brazilian journal of chemical engineering |
| Database: | PERIÓDICA |
| System number: | 000308933 |
| ISSN: | 0104-6632 |
| Authors: | Galesi, A.L.L1 Moraes, A.M Tamashiro, W.M.S.C2 |
| Institutions: | 1Universidade Estadual de Campinas, Faculdade de Engenharia Quimica, Campinas, Sao Paulo. Brasil 2Universidade Estadual de Campinas, Instituto de Biologia, Campinas, Sao Paulo. Brasil |
| Year: | 2004 |
| Season: | Abr-Jun |
| Volumen: | 21 |
| Number: | 2 |
| Pages: | 165-173 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental |
| English abstract | Due to the role of interleukin-2 (IL-2) in the mediation of immune response, this cytokine has been used in the treatment of some types of cancer and infectious diseases. However, relatively high levels of this cytokine are required to achieve significant activity. The aim of this work was to study a culture medium composition designed to increase the production of IL-2 by suspended murine EL-4 cells. The cultivations were carried out aiming at producing IL-2 in stirred bioreactors. The effects of concentration of glutamine, phorbol-12-myristate-13-acetate (PMA), concanavalin A (Con A), Pluronic F68, and fetal calf serum (FCS) on cell viability and IL-2 production were evaluated. PMA alone was more efficient in IL-2 production than it was in association with Con A. The maximum IL-2 production was around 162 ng/mL with 856 ng/mL PMA and 1.45% (v/v) FCS |
| Disciplines: | Química, Medicina, Biología |
| Keyword: | Química farmacéutica, Oncología, Biología celular, Citocinas, Interleucina, Cáncer, Biorreactores |
| Keyword: | Chemistry, Medicine, Biology, Medicinal chemistry, Oncology, Cell biology, Cytokines, Interleukin, Cancer, Bioreactors |
| Full text: | Texto completo (Ver HTML) |
