| Journal: | Brazilian journal of chemical engineering |
| Database: | PERIÓDICA |
| System number: | 000308893 |
| ISSN: | 0104-6632 |
| Authors: | Assis, O.B.G1 |
| Institutions: | 1Empresa Brasileira de Pesquisa Agropecuaria, Centro de Pesquisa e Instrumentacao, Sao Paulo. Brasil |
| Year: | 2003 |
| Season: | Sep |
| Volumen: | 20 |
| Number: | 3 |
| Pages: | 339-342 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Uniform attachment of enzymes to solid surfaces is essential in the development of bio and optical sensor devices. Immobilization by adsorption according to hydrophilic or hydrophobic nature is dependent on the charges and defects of the support surfaces. Sol-gel SiO2 densified glass surfaces, frequently used as supports for protein immobilization, are evaluated via scanning electron microscopy. The model protein is globular enzyme lysozyme, deposited by adsorption on functionalized surfaces. Formation of a protein layer is confirmed by FTIR spectroscopy, and the SEM images suggest discontinuous adsorption in areas where cracks predominate on the glass surface |
| Disciplines: | Química |
| Keyword: | Bioquímica, Biotecnología, Inmovilización enzimática, Sol-gel, Microscopía electrónica de barrido |
| Keyword: | Chemistry, Biochemistry, Biotechnology, Enzyme immobilization, Sol-gel, Scanning electron microscopy |
| Full text: | Texto completo (Ver HTML) |
