Immobilization and catalytic properties of lipase on chitosan for hydrolysis and esterification reactions



Document title: Immobilization and catalytic properties of lipase on chitosan for hydrolysis and esterification reactions
Journal: Brazilian journal of chemical engineering
Database: PERIÓDICA
System number: 000308891
ISSN: 0104-6632
Authors: 1

2
Institutions: 1Universidade Estadual de Maringa, Departamento de Engenharia Quimica, Maringa, Parana. Brasil
2Faculdade de Engenharia Quimica de Lorena, Departamento de Engenharia Quimica, Lorena, Sao Paulo. Brasil
Year:
Season: Dic
Volumen: 20
Number: 4
Pages: 343-355
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract The objective of this study was to evaluate the immobilization of lipase on a chitosan support by physical adsorption, aiming at its application in hydrolytic and synthetic reactions. Two types of chitosan (flakes and porous) were used for immobilizing lipase from a microbial source (Candida rugosa) and animal cells (porcine pancreas). The best results for recovery of total activity after immobilization were obtained for microbial lipase and porous chitosan beads. This set was selected for further immobilization studies, including full characterization of the immobilized derivative in aqueous and organic media. In aqueous medium, the operational and thermal stabilities of this preparation were quantified. In organic medium, the direct synthesis of n-butyl butyrate in organic solvent was chosen as a model reaction. The influence of several parameters, such as temperature, initial butyric acid concentration and amount of enzyme in the reaction system, was analyzed. Production of n-butyl butyrate was optimized and an ester yield response equation was obtained, making it possible to predict ester yields from known values of the three main factors. Use of this immobilized preparation was extended to the direct esterification of a large range of carboxylic acids (from C2 to C12) with a variety of alcohols (from C2 to C10)
Disciplines: Química
Keyword: Ingeniería química,
Biotecnología,
Enzimas inmovilizadas,
Lipasas,
Esterificación,
Hidrólisis
Keyword: Chemistry,
Chemical engineering,
Biotechnology,
Immobilized enzymes,
Lipases,
Esterification,
Hydrolysis
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