| Journal: | Brazilian journal of chemical engineering |
| Database: | PERIÓDICA |
| System number: | 000308646 |
| ISSN: | 0104-6632 |
| Authors: | Macedo, J.M.B1 Gottschalk, L.M.F2 Bon, E.P.S |
| Institutions: | 1Universidade do Estado do Rio de Janeiro, Instituto de Biologia Roberto Alcantara Gomes, Rio de Janeiro. Brasil 2Universidade Federal do Rio de Janeiro, Instituto de Quimica, Rio de Janeiro. Brasil |
| Year: | 1999 |
| Season: | Jun |
| Volumen: | 16 |
| Number: | 2 |
| Pages: | 163-169 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Lignin peroxidase (LiP) production has been extensively studied due to the potential use of this enzyme in environmental pollution control. Important aspects of the production of the enzyme by S. viridosporus T7A which have been studied include the improvement of yield and enzyme stabilization. In experiments performed in agitated flasks containing culture media composed of yeast extract as the source of nitrogen, mineral salts and different carbon sources, the use of glucose resulted in the highest values for LiP activity (350 U/L), specific LiP activity (450 U/g) and productivity (7 U/L/h). As the profile obtained with glucose-containing medium suggested enzyme instability, the effect of calcium carbonate was evaluated. The addition of CaCO3 in two different concentrations, 0.5% and 5.0%, resulted in higher values of maximum LiP activity, 600 and 900 U/L, respectively. The presence of this salt also anticipated enzyme activity peaks and allowed the detection of higher enzyme activities in the extracellular medium for longer periods of time. These results indicate a positive effect of calcium carbonate on LiP production, which is extremely relevant for industrial processes |
| Disciplines: | Química |
| Keyword: | Bioquímica, Biotecnología, Ingeniería química, Lignina peroxidasa, Carbonato de calcio, Actividad enzimática, Streptomyces viridosporus |
| Keyword: | Chemistry, Biochemistry, Biotechnology, Chemical engineering, Lignin peroxidase, Calcium carbonate, Enzymatic activity, Streptomyces viridosporus |
| Full text: | Texto completo (Ver HTML) |
