| Journal: | Brazilian journal of chemical engineering |
| Database: | PERIÓDICA |
| System number: | 000308905 |
| ISSN: | 0104-6632 |
| Authors: | Iannucci, N.B1 Wolman, F.J Camperi, S.A Cañizo, A.A.N Grasselli, M2 Cascone, O |
| Institutions: | 1Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Buenos Aires. Argentina 2Universidad Nacional de Quilmes, Departamento de Ciencia y Tecnología, Buenos Aires. Argentina |
| Year: | 2003 |
| Season: | Mar |
| Volumen: | 20 |
| Number: | 1 |
| Pages: | 27-32 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | High-performance affinity matrices were obtained by attaching pseudobiospecific ligands to hollow-fibre membranes. The neutral protease contained in FlavourzymeTM was purified to homogeneity with Yellow 4R-HE affinity hollow-fibre membranes. Immobilisation of Red HE-3B allowed purification of a milk-clotting enzyme obtained by solid-state culture of Mucor bacilliformis. Copper immobilisation through iminodiacetic acid allowed fractionation of Biocon Bioconcentrated PlusTM to separate the pectinesterase-containing fraction. The productivity of the developed processes - 1900, 94 and 750 U/ml.min, respectively - was 10- to 15-fold higher than that achieved with the same ligands immobilised on agarose-based soft gels, mainly due to the shortening of the purification processes |
| Disciplines: | Química |
| Keyword: | Bioquímica, Biotecnología, Purificación de proteínas, Cromatografía de afinidad, Ligandos pseudobioespecíficos |
| Keyword: | Chemistry, Biochemistry, Biotechnology, Protein purification, Affinity chromatography, Pseudobiospecific ligands |
| Full text: | Texto completo (Ver HTML) |
