| Journal: | Brazilian dental journal |
| Database: | PERIÓDICA |
| System number: | 000290223 |
| ISSN: | 0103-6440 |
| Authors: | Estrela, Carlos1 Ribeiro, Rosane Galhardo2 Estrela, Cyntia R.A3 Pecora, Jesus Djalma4 Sousa-Neto, Manoel Damiao5 |
| Institutions: | 1Universidade Federal de Goias, Faculdade de Odontologia, Goiania, Goias. Brasil 2Universidade de Ribeirao Preto, Faculdade de Odontologia, Ribeirao Preto, Sao Paulo. Brasil 3Universidade Federal de Goias, Faculdade de Odontologia, Goiania, Goias. Brasil 4Universidade de Sao Paulo, Faculdade de Odontologia, Ribeirao Preto, Sao Paulo. Brasil 5Universidade de Ribeirao Preto, Faculdade de Odontologia, Ribeirao Preto, Sao Paulo. Brasil |
| Year: | 2003 |
| Volumen: | 14 |
| Number: | 1 |
| Pages: | 58-62 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental |
| English abstract | The objective of this study was to analyze the antimicrobial effect of 2% sodium hypochlorite (NaOCl) and 2% chlorhexidine (CHX) by agar diffusion test and by direct exposure test. Five microorganisms: Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans, and one mixture of these were used. These strains were inoculated in brain heart infusion (BHI) and incubated at 37ºC for 24 h. For the agar diffusion test (ADT), 18 Petri plates with 20 ml of BHI agar were inoculated with 0.1 ml of the microbial suspensions, using sterile swabs that were spread on the medium, obtaining growth in junction. Fifty-four paper disks (9 mm in diameter) were immersed in the experimental solutions for 1 min. Subsequently, three papers disks containing one of the substances were placed on the BHI agar surface in each agar plate. The plates were maintained for 1 h at room temperature, and then incubated at 37ºC for 48 h. The diameter of microbial inhibition was measured around the papers disks containing the substances. For the direct exposure test, 162 #50 sterile absorbent paper points were immersed in the experimental suspensions for 5 min, and were then placed on Petri plates and covered with one of the irrigant solutions, or with sterile distilled water (control group). After intervals of 5, 10 and 30 min, the paper points were removed from contact with the solutions and individually immersed in 7 ml of Letheen Broth, followed by incubation at 37ºC for 48 h. Microbial growth was evaluated by turbidi |
| Disciplines: | Medicina |
| Keyword: | Odontología, Hipoclorito de sodio, Clorhexidina, Soluciones irrigantes, Intracanal, Antimicrobianos |
| Keyword: | Medicine, Dentistry, Sodium hypochlorite, Chlorhexidine, Irrigant solutions, Intracanal, Antimicrobial activity |
| Full text: | Texto completo (Ver HTML) |
