| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000388253 |
| ISSN: | 1516-8913 |
| Authors: | Reena1 Dhall, Purnima1 Kumar, Rita1 Kumar, Anil2 |
| Institutions: | 1Council of Scientific and Industrial Research, Institute of Genomics and Integrative Biology, Nueva Delhi, Delhi. India 2National Institute of Immunology, Nueva Delhi, Delhi. India |
| Year: | 2014 |
| Season: | Sep-Oct |
| Volumen: | 57 |
| Number: | 5 |
| Pages: | 803-809 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Nota breve o noticia |
| Approach: | Experimental |
| English abstract | Present study reports the validation (oxidation) of computationally predicted oxidation of xenobiotic contaminants by commercially available pure laccase from Trametes versicolor. Selected contaminants were predicted as potential targets for laccase oxidation by using in-silico docking tool. The oxidation by laccase was measured by change in absorbance at specific λ max of each compound. Sinapic acid and tyrosine were taken as positive and negative controls, respectively. Oxidation was observed in m-chlorophenol, 2,4 di-chlorophenol, 2,4,6 tri-chlorophenol, captan, atrazine and thiodicarb, except malathion, which showed no activity. It could be speculated that the predicted substrates showing oxidation shared homology at structural and chemical level with positive control compounds. In case of malathion, structural non-homology with sinapic acid could be attributed to its inactivity towards laccase that required further structural analysis. Thus, a remediation tool proposing an advanced remediation approach combining the application of theoretical in-silico method and subsequent experimental validation using pure laccase could be proposed. As number and type of xenobiotics increase, the unfeasibility to screen them experimentally for bioremediation also rise. This approach would be useful to reduce the time and cost required in other screening methods |
| Disciplines: | Biología |
| Keyword: | Ecología, Biotecnología, Xenobióticos, Contaminación ambiental, Biodegradación, Sustratos experimentales, Lacasa |
| Keyword: | Biology, Ecology, Biotechnology, Xenobiotics, Environmental pollution, Biodegradation, Experimental substrates, Laccase |
| Full text: | Texto completo (Ver HTML) |
