| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000372643 |
| ISSN: | 1516-8913 |
| Authors: | Lima, Francisco Esmaile de Sales1 Batista, Helena Beatriz Ruthner1 Campos, Fabricio Souza1 Kunert-Filho, Hiran Castagnino1 Cibulski, Samuel2 Finoketti, Fernando1 Arantes, Thalita1 Spilki, Fernando3 Roehe, Paulo Michel2 Rijsewijk, Franciscus Antonius Maria1 Franco, Ana Claudia1 |
| Institutions: | 1Universidade Federal do Rio Grande do Sul, Instituto de Ciencias Basicas da Saude, Porto Alegre, Rio Grande do Sul. Brasil 2Instituto de Pesquisas Veterinarias "Desiderio Finamor", Eldorado do Sul, Rio Grande do Sul. Brasil 3Universidade Feevale, Laboratorio de Microbiologia Molecular, Novo Hamburgo, Rio Grande do Sul. Brasil |
| Year: | 2014 |
| Season: | Ene-Feb |
| Volumen: | 57 |
| Number: | 1 |
| Pages: | 45-47 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental |
| English abstract | This study aimed to analyze the effect of the expression of Parainfluenza virus 5 (PIV5) V protein in bovine cells on the replication of Bovine herpesvirus 5 (BoHV-5). Growth properties of BoHV-5 were evaluated in parental and PIV5 transfected cells. In one-step growth experiments, the BoHV-5 reached higher titers at earlier time points in the transfected cells when compared to the parental cells. The mean plaque size produced by the BoHV-5 in transfected cells was larger than the parental cells. This indicated that the expression of the PIV5 V gene facilitated the release and cell-to-cell spread of BoHV-5 in bovine cells |
| Disciplines: | Biología, Química |
| Keyword: | Genética, Virus, Bioquímica, BoHV-5, V mRNA, Transfección |
| Keyword: | Biology, Chemistry, Genetics, Virus, Biochemistry, BoHV-5, V mRNA, Transfection |
| Full text: | Texto completo (Ver PDF) |
