| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000373467 |
| ISSN: | 1516-8913 |
| Authors: | Sharmin, Shamima Akhtar1 Alam, Md. Jahangir3 Sheikh, Md. Mominul Islam4 Sarker, Kanak Kanti2 Khalekuzzaman, Muhammad5 Haque, Md. Anwarul6 Alam, Mohammad Firoz2 Alam, Iftekhar1 |
| Institutions: | 1Gyeongsang National University, Division of Applied Life Sciences, Jinju. Corea del Sur 2University of Rajshahi, Department of Botany, Rajshahi. Bangladesh 3Shizuoka University, Graduate School of Science and Technology, Shizuoka. Japón 4Gyeongsang National University, Jinju. Corea del Sur 5University of Rajshahi, Rajshahi. Bangladesh 6Islamic University, Department of Biotechnology and Genetic Engineering, Kushtia. Bangladesh |
| Year: | 2014 |
| Season: | May-Jun |
| Volumen: | 57 |
| Number: | 3 |
| Pages: | 394-401 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | In this work, plant regeneration via somatic embryogenesis was achieved from leaf and internode derived callus of Wedelia calendulacea, an endangered medicinal plant. Primary callus was induced by culturing leaf disc and internode explant on Murashige and Skoog medium supplemented with 2.0 mg L-1 of 2,4-D under light condition. Transfer of embryogenic callus on a reduced concentration of 2,4-D facilitated somatic embryo development while calluses remained unorganized at the same 2,4-D level. A histological analysis confirmed somatic embryo by revealing the presence of a closed vascular system in the developing embryos and lack of a vascularconnection with surrounding callus tissues. Somatic embryos germinated into plantlets upon transfer on MS medium containing 1.0 mg L-1 BAP plus 0.5 mg L-1 GA3. Plantlets were acclimatized successfully and survived under soil condition. This is the first on somatic embryogenesis of W.calendulacea. This result could facilitate genetic transformation of this important medicinal plant |
| Disciplines: | Biología |
| Keyword: | Angiospermas, Fisiología vegetal, Plantas medicinales, Micropropagación, Embriogénesis somática, Medios de cultivo, Wedelia calendulacea, Asteraceae |
| Keyword: | Biology, Angiosperms, Plant physiology, Medicinal plants, Micropropagation, Somatic embryogenesis, Culture media, Wedelia calendulacea, Asteraceae |
| Full text: | Texto completo (Ver HTML) |
