Separation of polyhydroxyalkanoates-producing bacterial strains using PHA synthase gene and their evaluation for PHA deposition
Document title: Separation of polyhydroxyalkanoates-producing bacterial strains using PHA synthase gene and their evaluation for PHA deposition
Journal: Brazilian archives of biology and technology
Database: PERIÓDICA
System number: 000365608
ISSN: 1516-8913
Authors: 1
2
1
2
1
2
3
Institutions: 1PCSIR Laboratories Complex, Food and Marine Resources Research Centre, Karachi, Baluchistán. Pakistán
2PCSIR Laboratories Complex, Applied Chemistry Research Centre, Karachi, Baluchistán. Pakistán
3PCSIR Laboratories Complex, Head Office, Islamabad. Pakistán
Year:
Season: Jul-Ago
Volumen: 56
Number: 4
Pages: 645-652
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract In this study, a variety of samples were screened for the presence of PHA synthase gene. Results showed that 16 out of 102 isolated were positive for PHA respective genes. The highest prevalence was observed in Pseudomonas aeruginosa. The capability of PHA production was also shown by growing these strains on the defined medium and subsequent analysis using intracellular granules staining and Fourier transform infrared spectroscopy (FT-IR). The microscopic analysis showed that the positive strains accumulated PHA in the cell. The FT-IR analysis showed the presence of PHA peaks in the dried cells as well as in extraction product. P aeruginosa strain P7 showed higher concentration of PHA compared to the others as demonstrated by the highest respective peaks in FT-IR
Disciplines: Química
Keyword: Bioquímica,
Polímeros biodegradables,
Polihidroxialcanoatos,
Genes,
Pseudomonas aeruginosa
Keyword: Chemistry,
Biochemistry,
Biodegradable polymers,
Polyhydroxyalkanoates,
Genes,
Pseudomonas aeruginosa
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