| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000337395 |
| ISSN: | 1516-8913 |
| Authors: | Jose, Anderson Cleiton1 Silva, Edvaldo Aparecido Amaral da2 Davide, Antonio Claudio1 Toorop, Peter3 |
| Institutions: | 1Universidade Federal de Lavras, Departamento de Ciencias Florestais, Lavras, Minas Gerais. Brasil 2Universidade Estadual Paulista "Julio de Mesquita Filho", Faculdade de Ciencias Agronomicas, Botucatu, Sao Paulo. Brasil 3Royal Botanic Gardens, Seed Conservation Department, Londres. Reino Unido |
| Year: | 2011 |
| Season: | May-Jun |
| Volumen: | 54 |
| Number: | 3 |
| Pages: | 465-476 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, analítico |
| English abstract | The effect of seed drying and imbibition was studied by differential protein expression using two dimensional gel electrophoresis. After drying to a range of water contents, seeds were germinated to assess the viability. Seeds of Magnolia ovata did not withstand the desiccation down to 0.10 g H2O · g-1 dw. The critical water content below which the desiccation sensitivity became apparent was around 0.18 g H2O · g-1 dw (-26.5 MPa). Total protein was extracted and separated by 2D electrophoresis from fresh seeds (0.28 g H2O · g-1 dw), mild dried seeds (0.25 g H2O · g-1 dw) and seeds at low water content (0.10 g H2O · g-1 dw) before and after imbibition for 10 days. The proteome profile revealed the presence of 588 spots on each silver stained gel, from which 21 showed differential expression, correlated with desiccation and germination, by increased or decreased expression. After MS/MS sequencing, three protein spots produced spectra that matched to a Magnolia salicifolia legumin precursor. Results suggested an involvement of this protein in the events taking place during the drying and subsequent imbibitions of the dried seeds |
| Disciplines: | Biología |
| Keyword: | Fisiología vegetal, Genética, Fitoquímica, Silvicultura, Magnolia ovata, Expresión proteica, Proteómica, Desecación, Germinación |
| Keyword: | Biology, Genetics, Plant physiology, Phytochemistry, Silviculture, Magnolia ovata, Protein expression, Desiccation, Proteomics |
| Full text: | Texto completo (Ver HTML) |
