| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000392557 |
| ISSN: | 1516-8913 |
| Authors: | Lopes, Leandra Fiori1 Guembarovski, Roberta Losi1 Guembarovski, Alda Losi2 Kishima, Marina Okuyama2 Campos, Clodoaldo Zago3 Derossi, Daniela Rudgeri2 Ariza, Carolina Batista1 Ozawa, Patricia Midori Murobushi1 Oliveira, Carlos Eduardo Coral de1 Banin-Hirata, Bruna Karina1 Vitiello, Glauco Akelinghton Freire1 Borelli, Sueli Donizete4 Watanabe, Maria Angelica Ehara1 |
| Institutions: | 1Universidade Estadual de Londrina, Laboratorio de Estudos e Aplicacoes de Polimorfismos de DNA, Londrina, Parana. Brasil 2Universidade Estadual de Londrina, Departamento de Patologia, Analises Clínicas e Toxicologicas, Londrina, Parana. Brasil 3Universidade Estadual de Londrina, Departamento de Clinica Medica, Londrina, Parana. Brasil 4Universidade Estadual de Maringa, Departamento de Analises Clinicas, Maringa, Parana. Brasil |
| Year: | 2014 |
| Season: | Nov-Dic |
| Volumen: | 57 |
| Number: | 6 |
| Pages: | 895-899 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | A subgroup of tumor that has received attention is triple-negative breast cancer (TNBC), which present s phenotype of negative estrogen receptor, negative progesteron e receptor and has no overexpression of HER2. TP53 acts as a tumor suppressor limiting the proliferation of dama ged cells. A polymorphic site (rs1042522) of TP53 encodes either an arginine or a proline amino acid, but its biological significance remains unclear. This stud y aimed to investigate this variant and its expression in search for a possible involvement in TNBC susceptibilit y and clinical outcome. Genetic polymorphism was evaluated in 50 p atients and 115 controls by PCR based methodology a nd immunohistochemistry was done with monoclonal antibody. Case-control study showed no positive or negative association (OR= 0.95; CI95%= 0.48-1.89). Comparison of genotypes and clinical outcome showed no signi ficant results. Despite most of patients presented p53 positive staining by immunohistochemistry, there was n o significant association in relation to prognostic parameters. R esults demonstrated a lack of association between c odon 72 polymorphism, susceptibility and prognosis of TNBC. Immunohistochemistry analysis should be done more carefully, since most of the patients had the somat ic mutation of p53, which could be an indicator of prognostic value in TNBC |
| Disciplines: | Química, Medicina |
| Keyword: | Bioquímica, Genética, Inmunología, Cáncer, Mama, Polimorfismo genético, Inmunohistoquímica |
| Keyword: | Chemistry, Medicine, Biochemistry, Genetics, Immunology, Breast, Cancer, Genetic polymorphism, Immunohistochemistry |
| Full text: | Texto completo (Ver PDF) |
