| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000373459 |
| ISSN: | 1516-8913 |
| Authors: | Monte, Leonardo Garcia1 Leal, Fernanda Munhoz Dos Anjos1 Hartwig, Daiane Drawanz1 Vasconcellos, Sílvio Arruda2 Brihuega, Bibiana3 Dellagostin, Odir Antonio1 Hartleben, Claudia Pinho1 |
| Institutions: | 1Universidade Federal de Pelotas, Centro de Desenvolvimento Tecnologico, Pelotas, Rio Grande do Sul. Brasil 2Universidade de Sao Paulo, Faculdade de Medicina Veterinaria e Zootecnia, Sao Paulo. Brasil 3Instituto Nacional de Tecnología Agropecuaria, Centro de Ciencias Veterinarias y Agronómicas, Buenos Aires. Argentina |
| Year: | 2014 |
| Season: | May-Jun |
| Volumen: | 57 |
| Number: | 3 |
| Pages: | 357-360 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | The production of recombinant LipL32 protein using Escherichia coli has been used extensively for the development of vaccines and diagnostic tests for leptospirosis. However, E. coli has demonstrated limitations, including low yield and lack of post-translational modifications. In this study, rLipL32 was produced in eukaryotic expression system (Pichia pastoris) and evaluated the antigen by enzyme-linked immunosorbent assay (ELISA). The yield obtained from the culture supernatant reached 270 mg/L and ELISA showed an accuracy of 95.34%. In summary, the production of rLipL32 using P. pastoris did not impair the antigenic characteristics of this antigen and ensured its use for detecting the leptospiral antibodies in swine sera |
| Disciplines: | Biología, Química |
| Keyword: | Bioquímica, Bioquímica clínica, Proteínas recombinantes, Pruebas diagnósticas, Pichia pastoris, Leptospirosis, ELISA |
| Keyword: | Biology, Chemistry, Biochemistry, Clinical biochemistry, Recombinant proteins, Diagnostic tests, Pichia pastoris, Leptospirosis, ELISA |
| Full text: | Texto completo (Ver HTML) |
