| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000362765 |
| ISSN: | 1516-8913 |
| Authors: | Lakshmi, Subbaiah Revathi1 Benjamin, Jambarapu Herald Franklin1 Kumar, Tirupathi Senthil2 Murthy, Garimella Venkata Suryanarayana3 Rao, Mandali Venkateswara1 |
| Institutions: | 1Bharathidasan University, Department of Plant Science, Tiruchirapalli, Tamil Nadu. India 2Bharathidasan University, Department of Industry University Collaboration, Tiruchirapalli, Tamil Nadu. India 3Botanical Survey of India, Coimbatore, Tamil Nadu. India |
| Year: | 2013 |
| Season: | May-Jun |
| Volumen: | 56 |
| Number: | 3 |
| Pages: | 421-430 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | An efficient system was developed for indirect plant regeneration from in vitro-derived leaf and internode explants of Hoya wightii ssp. palniensis. Maximum percentage of the organogenic callus was obtained on MS medium supplemented with NAA (1.0 mg/l) and 2,4-D (2.0 mg/l). The best shoot bud induction was observed on MS medium with BA (1.0 mg/l) +IBA (0.5 mg/l). The coconut water (15%) was better, resulting in a differentiation of the shoot initials in to well-developed shoots. The elongated shoots (› 3cm long) were rooted on a full strength MS basal medium, supplemented with 0.2 mg/l of IBA. Finally, the rooted plants were transferred to the soil with 80% success rate. This protocol was utilized for the in vitro propagation of this endangered plant species |
| Disciplines: | Biología |
| Keyword: | Angiospermas, Fisiología vegetal, Cultivo de tejidos, Organogénesis, Reguladores del crecimiento, Propagación vegetativa, Hoya wightii, Asclepiadaceae |
| Keyword: | Biology, Angiosperms, Plant physiology, Tissue culture, Organogenesis, Growth regulators, Vegetative propagation, Hoya wightii, Asclepiadaceae |
| Full text: | Texto completo (Ver HTML) |
