| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000388218 |
| ISSN: | 1516-8913 |
| Authors: | Bajaj, Bijender Kumar1 Singh, Satbir1 Khullar, Mowkshi1 Singh, Kaurab1 Bhardwaj, Shikha1 |
| Institutions: | 1University of Jammu, School of Biotechnology, Jammu, Kashmir. India |
| Year: | 2014 |
| Season: | Sep-Oct |
| Volumen: | 57 |
| Number: | 5 |
| Pages: | 653-662 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Nota breve o noticia |
| Approach: | Experimental |
| English abstract | The aim of this work was to study the production of fibrinolytic protease by Bacillus subtilis I-2 on agricultural residues. Molasses substantially enhanced (63%) protease production (652.32 U/mL) than control (398.64 U/mL). Soybean meal supported maximum protease production (797.28 U/mL), followed by malt extract (770.1 U/mL), cotton cake (761.04 U/mL), gelatin (742.92 U/mL) and beef extract (724.8 U/mL). Based on the Plackett-Burman designed experiments, incubation time, soybean meal, mustard cake and molasses were identified as the significant fermentation parameters. Ammonium sulfate precipitation and DEAE sephadex chromatography resulted 4.8-fold purification of protease. Zymography showed the presence of three iso-forms in the partially purified protease preparation, which was confirmed by the SDS-PAGE analysis (42, 48, 60 kDa). Protease exhibited maximum activity at 50oC and at pH 8.0. Significant stability was observed at 30-50oC and at pH 7.0-10.0. Mg2+, Zn2+, Co2+, Ca2+, Mn2+ and Cu2+ , EGTA, EDTA and aprotinin severely decreased the enzyme activity |
| Disciplines: | Biología, Química |
| Keyword: | Fermentaciones, Biotecnología, Residuos agrícolas, Bioproducción, Proteasa fibrinolítica, Bacillus subtilis |
| Keyword: | Biology, Chemistry, Fermentation, Biotechnology, Agricultural wastes, Bioproduction, Fibrinolytic protease, Bacillus subtilis |
| Full text: | Texto completo (Ver HTML) |
