| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000331985 |
| ISSN: | 1516-8913 |
| Authors: | Tang, Xiaobo1 Zhang, Rong1 Wang, Zhigang1 Yu, Lei1 Li, Shanshan1 Zhu, Daling1 |
| Institutions: | 1Harbin Medical University, College of Pharmacy, Harbin, Heilungkiang. China |
| Year: | 2008 |
| Season: | Mar-Abr |
| Volumen: | 51 |
| Number: | 2 |
| Pages: | 281-286 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Extracellular Ca2+ influx was blocked by L-type Ca2+ channel blocker nifedipine to observe the effects of 15-hydroxyeicosatetraenoic acid on the constriction of rabbit pulmonary artery rings and on the changes of Ca2+ level in the rabbit pulmonary artery smooth muscle cells, and further to investigate the mechanism of the calcium mobilization induced by the 15-HETE under hypoxic conditions. The effect of extracellular Ca2+ on tension of the rabbit PA rings was also studied. Nifedipine (10 µ mol/L) had no effect on 1 µ mol/L 15-hydroxyeicosatetraenoic acid induced vasoconstriction under normoxic and hypoxic conditions. Intracellular Ca2+ increased markedly in the 15-HETE group (cells were exposed to 1 µ mol/L 15-HETE for 8 min during culture) compared to the control group (P < 0.05). The study demonstrated that the 15-HETE could induce the elevation of Ca2+ in the pulmonary artery smooth muscle cells and the elevated calcium came from the release of the intracellular calcium |
| Disciplines: | Medicina |
| Keyword: | Medicina experimental, Fisiología celular, Flujo de calcio, Acido 15-hidroxieicosatetraenoico, Arteria pulmonar, Contracción muscular, Músculo liso |
| Keyword: | Medicine, Experimental medicine, 15-hydroxyeicosatetraenoic acid, Calcium flux, Pulmonary artery, Muscle contraction, Smooth muscle |
| Full text: | Texto completo (Ver HTML) |
