| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000392538 |
| ISSN: | 1516-8913 |
| Authors: | Carvalho, Milene Alves de Figueiredo1 Paiva, Renato2 Stein, Vanessa Cristina3 Herrera, Raírys Cravo4 Porto, Jorge Marcelo Padovani5 Vargas, Daiane Peixoto6 Alves, Eduardo7 |
| Institutions: | 1Empresa Brasileira de Pesquisa Agropecuaria, Cafe, Brasilia, Distrito Federal. Brasil 2Universidade Federal de Lavras, Departamento de Biologia, Lavras, Minas Gerais. Brasil 3Universidade Federal de Goias, Jatai, Goias. Brasil 4Universidade Federal do Para, Altamira, Para. Brasil 5Instituto Federal do Espirito Santo, Nova Venecia, Espirito Santo. Brasil 6Empresa Brasileira de Pesquisa Agropecuaria, Clima Temperado, Pelotas, Rio Grande do Sul. Brasil 7Universidade Federal de Lavras, Departamento de Fitopatologia, Lavras, Minas Gerais. Brasil |
| Year: | 2014 |
| Season: | Nov-Dic |
| Volumen: | 57 |
| Number: | 6 |
| Pages: | 851-859 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | This work studied a new protocol for organogenic calli induction and characterization of the morpholog y and ultrastructure of callogenesis in leaf explants of Passiflora gibertii N. E. Brown, a native passion fruit species from Brazil. Calli induction was performed in different growth conditions (light and dark), different MS medium salt concentrations (MS and MS half strength) and the presence or absence of coconut water. The leaf explants maintained in the dark were more responsive to bud formation. In order to reduce spending on in vitro culture, the most suitable induction medium for P. gibertii organogenesis could, therefore be the MS half strength salt concentration medium maintained in the dark. The addition of coconut water to the culture medium was essential for both calli induction and bud formation. The morphological and ultrastructural features of the organogenic calli were isodiametric cells, characterized by an organized cellular system, nucleus with prominent nucleoli, presence of starch grains and dense cytoplasm rich in endoplasmic reticulum. The scanning electron microscopy demo nstrated that buds were present on these calli |
| Disciplines: | Biología, Agrociencias |
| Keyword: | Biología celular, Fisiología vegetal, Fitotecnia, Passifloraceae, Callogénesis, Electromicroscopía, Microscopía óptica, Cultivo de tejidos |
| Keyword: | Biology, Agricultural sciences, Cell biology, Plant physiology, Crop husbandry, Passifloraceae, Callogenesis, Electromicroscopy, Optic microscopy, Tissue culture |
| Full text: | Texto completo (Ver PDF) |
