| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000392542 |
| ISSN: | 1516-8913 |
| Authors: | Bakar, Dalilah Abu1 Ahmed, Bakrudeen Ali1 Taha, Rosna Mat1 |
| Institutions: | 1University of Malaya, Institute of Biological Sciences, Kuala, Lumpur. Malasia |
| Year: | 2014 |
| Season: | Nov-Dic |
| Volumen: | 57 |
| Number: | 6 |
| Pages: | 860-866 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | Celosia argentea (Var.) cristata (Amaranthaceae) is a widely cultivated ornamental plant, which has antibacterial, astringent, haemostatic, hypertensive, ophthalmic, and parasitic significance. This study describes a protocol for in vitro callus induction and plant regeneration from leaf and stem explants of C. argentea using Murashige and Skoog (MS) medium. Callus culture was initiated and established from seedling, leaf, and stem explants. Explants were cultured on MS medium supplemented with auxin alone (0.5 mg/L Naphthaleneacetic acid (NAA), 2, 4- Dicholorophenoxyacetic acid (2, 4-D). Green and red compact callus (98%) were induced using MS medium supplemented with 0.5 mg/L NAA and 1.0 mg/L Benzyla denine (BA). Two different concentrations (1.0 mg/L BA + 0.5 mg/L NAA and 1.0 mg/L BA + 1.0 mg/L NAA) succes sfully induced plant regeneration with multiple shoots (1.5 and 0.9 shoots per explants, respectively). Successful shoots were transferred to rooting medium supplemented with 1.0 mg/L Indole-3-acetic acid (IAA) (80%) at 35 th day. Acclimatization was done, which resulted in 9 0% of the plantlets surviving in garden soil. This protocol could be used to micropropagate C. argentea for conservation, commercial natural product production. The high frequency of callus indicated potential of C. argentea for secondary metabolite production (celosin) in pharma ceutical industry |
| Disciplines: | Agrociencias |
| Keyword: | Fitotecnia, Plantas ornamentales, Plantas medicinales, Fisiología vegetal, Celosia argentea, Amaranthaceae, Cultivo de tejidos, Micropropagación, Callos, Regeneración vegetal |
| Keyword: | Agricultural sciences, Crop husbandry, Ornamental plants, Medicinal plants, Plant physiology, Celosia argentea, Amaranthaceae, Tissue culture, Micropropagation, Callus, Plant regeneration |
| Full text: | Texto completo (Ver PDF) |
