| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000391511 |
| ISSN: | 1516-8913 |
| Authors: | Carneiro, Lara Aparecida Buffoni Campos1 Costa-Silva, Tales Alexandre1 Souza, Claudia Regina Fernandes1 Bachmann, Luciano2 Oliveira, Wanderley Pereira1 Said, Suraia1 |
| Institutions: | 1Universidade de Sao Paulo, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Ribeirao Preto, Sao Paulo. Brasil 2Universidade de Sao Paulo, Faculdade de Filosofia, Ciencias e Letras de Ribeirao Preto, Ribeirao Preto, Sao Paulo. Brasil |
| Year: | 2014 |
| Season: | Jul-Ago |
| Volumen: | 57 |
| Number: | 4 |
| Pages: | 578-586 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | This work studied the immobilization of Cercospora kikuchii lipases on chitosan microparticles by chemical attachment on chitosan acetate microparticles activated by glutaraldehyde (CAM) added before or after the enzyme and physical adsorption on highly deacetylated chitosan hydrochloride microparticles (CHM). Lipases covalently immobilized on pre-activated CAM showed better performance retaining 88.4% of the enzymatic activity, with 68.2% of immobilization efficiency (IE). The immobilized enzyme retained an activity of about 53.5 % after five reuses, using p-NPP as substrate. Physical adsorption of lipase onto highly deacetylated CHM showed 46.2 % of enzymatic activity and 28.6% of IE. This immobilized derivative did not lose activity up to 80 days of storage at 4°C, while lipases immobilized on pre-activated CAM maintained its activity up to 180 days at same conditions. Taken together the results indicate that chitosan microparticles provide an optimal microenvironment for the immobilized enzyme to maintain good activity and stability |
| Disciplines: | Biología, Química |
| Keyword: | Hongos, Bioquímica, Biotecnología, Lipasas, Inmovilización enzimática, Cercospora kikuchii |
| Keyword: | Biology, Chemistry, Fungi, Biochemistry, Biotechnology, Lipases, Enzyme immobilization, Cercospora kikuchii |
| Full text: | Texto completo (Ver HTML) |
