| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000387703 |
| ISSN: | 1516-8913 |
| Authors: | Pang, Guangchang1 Wang, Yufang1 Xie, Junbo1 Chen, Qingsen1 Hu, Zhihe1 |
| Institutions: | 1Tianjin University of Commerce, Tianjin Key Laboratory of Food Biotechnology, Tianjin. China |
| Year: | 2015 |
| Season: | May-Jun |
| Volumen: | 58 |
| Number: | 3 |
| Pages: | 287-294 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | FcRn (neonatal Fc receptor) plays an important role in IgG transportation, antigen presentation and signal transmission. In this study, the complement fixation test and flow cytometry test were performed to verify whether the heterologous antibody could be transmitted to the serum or leukocyte with FcγR (Fc gamma receptor) across the intestinal mucosa. The results showed that rabbit anti-bovine IgG could be detected in both the serum and the leukocytes, which indicated that the heterologous antibody could transport across the intestinal mucosa to enter the blood and be effectively delivered to the leukocytes with FcγR. In addition, the results also showed that the rabbit anti-bovine IgG still could be detected in the leukocyte group (P=0.044<0.05) after 21 days. It indicated that the rabbit IgG could exist in the body for a long term (up to 21 days) after being transported to the cells containing FcγR |
| Disciplines: | Medicina, Química |
| Keyword: | Bioquímica, Inmunología, Biología celular, IgG, Transporte celular, Barrera gastrointestinal, Receptor neonatal Fc, Prueba de fijación del complemento, Citometría de flujo |
| Keyword: | Medicine, Chemistry, Biochemistry, Immunology, Cell biology, IgG, Cell transport, Gastrointestinal barrier, Neonatal Fc receptor, Complement fixation test, Flow cytometry |
| Full text: | Texto completo (Ver HTML) |
