| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000374393 |
| ISSN: | 1516-8913 |
| Authors: | Rahulan, Raji1 Manjunath, G1 Nampoothiri, Kesavan Madhavan1 Pandey, Ashok1 |
| Institutions: | 1National Institute for Interdisciplinary Science and Technology, Biotechnology Division, Kerala. India |
| Year: | 2014 |
| Season: | Mar-Abr |
| Volumen: | 57 |
| Number: | 2 |
| Pages: | 187-193 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental |
| English abstract | A bioprocess was developed for extracellular MAP production from Streptomyces gedanensis by solid-state fermentation. Response surface methodology of Box Behken Design was performed to evaluate the interaction effects of most significant variables {inoculum size, (NH4)2SO4 concentration, MgSO4.7H2O and tryptone) on MAP production after the single parameter optimization and it resulted a maximum MAP production of 55.26 IU/g PUF after 120 h of fermentation. The concentrated crude MAP displayed a pH and temperature optimum of 8.5 and 50°C. By analyzing the thermal stability, the MAP was found to be stable in a temperature range of 50 to 55°C but lost about 50% of its activity at 65°C after 30 min. This is a first report of this kind of study for MAP |
| Disciplines: | Biología, Química |
| Keyword: | Bacterias, Bioquímica, Fermentaciones, Streptomyces gedanensis, Metionina amino peptidasa, Fermentación, Diseño de Box-Behnken |
| Keyword: | Biology, Chemistry, Bacteria, Biochemistry, Fermentation, Fermentations, Streptomyces gedanensis, Methionine amino peptidase, Box-Behnken design |
| Full text: | Texto completo (Ver PDF) |
