| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000374407 |
| ISSN: | 1516-8913 |
| Authors: | Leitao, Cintia Camurca Fernandes1 Costa, Jose Jackson do Nascimento1 Brito, Ivina Rocha2 Magalhaes-Padilha, Deborah de Melo2 Almeida, Anderson Pinto2 Figueiredo, José Ricardo de2 van den Hurk, Robert3 Silva, Jose Roberto Viana1 |
| Institutions: | 1Utrecht University, Department of Pathobiology, Utrecht. Países Bajos |
| Year: | 2014 |
| Season: | Mar-Abr |
| Volumen: | 57 |
| Number: | 2 |
| Pages: | 200-208 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental |
| English abstract | The aim of the present study was to determine the role of GDF-9 and/or FSH on the growth and mRNA expression for FSH-R, GDF-9, and BMPs in goat secondary follicles after culture in vitro. Goat secondary follicles (~200µm) were isolated and cultured for six days in minimum essential medium (MEM) supplemented with GDF-9 (200 ng/mL), FSH (50 ng/mL) or both. At the beginning and end of culture, the follicular diameter was evaluated and compared. The levels of mRNA for GDF-9, FSH-R and BMPs -2, -4, -6, -7 and -15 in cultured follicles were quantified by real time PCR. The results showed that a significant increase of follicle diameter after six days when compared to day 0, but the presence of GDF-9 and FSH did not influence the follicular growth in comparison with those cultured in MEM. Real time PCR showed that GDF-9 down-regulated the levels of mRNA for BMPs -2 and -15, while FSH either alone or in combination with GDF-9 did not affect the expression of GDF-9, FSH-R and BMPs. In conclusion, GDF-9 reduced the expression of BMP-2 and -15 in caprine preantral follicles after their culture, but FSH either alone or in association with GDF-9 did not control the expression of GDF-9, FSH-R and BMPs |
| Disciplines: | Biología, Química, Medicina veterinaria y zootecnia |
| Keyword: | Biología celular, Bioquímica, Reproducción y genética animal, BMPs, Folículos, GDF-9, In vitro, ARNm |
| Keyword: | Biology, Chemistry, Veterinary medicine and animal husbandry, Cell biology, Biochemistry, Animal reproduction and genetics, BMPs, Follicles, GDF-9, In vitro, mRNA |
| Full text: | Texto completo (Ver PDF) |
