| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000387711 |
| ISSN: | 1516-8913 |
| Authors: | Li, Ying Xia1 Zhao, Feng Juan2 Yu, Dong Dong3 |
| Institutions: | 1Qingdao Agricultural University, Marine Science and Engineering College, Qingdao, Shandong. China 2BinZhou University, Department of Biological Sciences, Binzhou, Shandong. China 3Huangdao Entry-Exit Inspection and Quarantian Bureau, Qingdao, Shandong. China |
| Year: | 2015 |
| Season: | May-Jun |
| Volumen: | 58 |
| Number: | 3 |
| Pages: | 462-467 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | The influence of nitrogen (N) limitation on the cell growth, chlorophyll content, intracellular lipid production and expression levels of two pathway genes in Chlorella sorokiniana was investigated in mixotrophic culture in this study. The maximum biomass concentration of C. sorokinianacultured in modified BG11 medium containing 3, 6, 9, 12 and 15 mM N were 1.60, 2.21, 2.74, 3.18 and 3.21 g/L of dry cell weight, respectively with maximum specific growth rate of 0.180, 0.198, 0.201, 0.203, 0.206 day−1 during culture with an initial N feed of 3, 6, 9, 12 and 15 mM in the first eight days, respectively. The maximum lipid content was 51 % with 3 mM N. However, the maximum lipid productivity of 0.00884 g L−1 day−1 was achieved with 6 mM N. Expression levels of accD (heteromeric acetyl-CoA carboxylase beta subunit) and accI (homomeric acetyl-CoA carboxylase) genes in C. sorokiniana were studied by real-time PCR. Increased expression levels of accD reflected the increased lipid content in stationary phase. In contrast, expression of the acc1 gene always remained low, showing that the gene might not be critical for lipid accumulation |
| Disciplines: | Biología |
| Keyword: | Algas, Fisiología vegetal, Cultivo de algas, Limitación de sustrato, Nitrógeno, Crecimiento celular, Lípidos, Expresión génica, Chlorella sorokiniana, Chlorophyta |
| Keyword: | Biology, Algae, Plant physiology, Algal culture, Substrate limitation, Nitrogen, Cell growth, Lipids, Gene expression, Chlorella sorokiniana, Chlorophyta |
| Full text: | Texto completo (Ver HTML) |
