| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000387706 |
| ISSN: | 1516-8913 |
| Authors: | Lima, Eleonidas Moura1 Lopes, Otavio Sergio3 Soares, Leonardo Ferreira4 Arruda, Talitta Dantas2 Gigek, Carolina Oliveira5 Melo, Cynthia Germoglio Farias2 Smith, Marilia Arruda Cardoso5 Oliveira, Joao Ricardo Goncalves2 Medeiros, Arnaldo6 Delatorre, Plínio2 Burbano, Rommel Rodriguez7 |
| Institutions: | 1Universidade Federal da Paraiba, Departamento de Biologia Molecular, Joao Pessoa, Paraiba. Brasil 2Universidade Federal da Paraiba, Laboratorio de Biologia Molecular Estrutural e Oncogenetica, Joao Pessoa, Paraiba. Brasil 3Clinica Dermatologica Santa Catarina, Departamento de Dermatologia, Joao Pessoa, Paraiba. Brasil 4Universidade Estadual da Paraiba, Departamento de Farmacia, Campina Grande, Paraiba. Brasil 5Universidade Federal de Sao Paulo, Departamento de Genetica de Morfologia, Sao Paulo. Brasil 6Universidade Federal da Paraiba, Departamento de Medicina, Joao Pessoa, Paraiba. Brasil 7Universidade Federal do Para, Instituto de Ciencias Biologicas, Belem, Para. Brasil |
| Year: | 2015 |
| Season: | May-Jun |
| Volumen: | 58 |
| Number: | 3 |
| Pages: | 414-420 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Gastric cancer (GC) is a multifactorial disease with a high mortality rate in Brazil and worldwide. This work aimed to evaluate single nucleotide polymorphisms (SNP) rs1695, in the Glutathione S-Transferase Pi (GSTP1) gene in GC samples by comparative analysis Specific PCR - ASP and Dideoxy Single Allele-Specific PCR - DSASP methods. The DSASP is the proposed new method for allelic discrimination. This work analyzed 60 GC samples, 26 diffuse and 34 intestinal types. The SNP rs1695 of the GSTP1 gene was significantly associated with GC analyzed by DSASP method (χ2 = 9.7, P < 0.05). A comparative analysis of the data obtained from both methods did not differ significantly (χ2 = 0.08, P > 0.05). These results suggest that the SNP rs1695 of the GSTP1 gene was a risk factor associated with gastric carcinogens is and the DSASP method was a new successfully low-cost strategy to study allelic discrimination |
| Disciplines: | Medicina |
| Keyword: | Oncología, Genética, Cáncer gástrico, Polimorfismos de nucleótido único (SNPs), Discriminación alélica, Genotipificación |
| Keyword: | Medicine, Oncology, Genetics, Gastric cancer, Single nucleotide polymorphism (SNPs), Allelic discrimination, Genotyping |
| Full text: | Texto completo (Ver HTML) |
