| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000374403 |
| ISSN: | 1516-8913 |
| Authors: | Ismail, Mohd Iswadi1 Osman, Khairul2 Ibrahim, Siti Fatimah1 Jaafar, Farah Hanan Fatihah1 Ghani, Nur Azianie Abd1 Zainalabidin, Fazly Ann3 Othman, Abas Mazni4 |
| Institutions: | 1The National University of Malaysia, Department of Physiology, Kuala, Lumpur. Malasia 2The National University of Malaysia, Faculty of Health Sciences, Kuala, Lumpur. Malasia 3Veterinary Research Institute, Department of Veterinary Services, Ipoh, Perak. Malasia 4Malaysian Agricultural Research and Development Institute, Serdang, Selangor. Malasia |
| Year: | 2014 |
| Season: | Mar-Abr |
| Volumen: | 57 |
| Number: | 2 |
| Pages: | 194-199 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental |
| English abstract | The objective of this study was to evaluate the potential of using single tubing-type canister on sperm quality. Semen was collected from the Bali cattle bull by electroejaculation technique and was cryopreserved in liquid nitrogen using slow freezing cryopreservation method. Two type of canister volume was used in this study; commercial canister (342.25π x 278 mm2) and single tubing-type canister (4π x 90 mm2). Makler counting chamber and computer assisted sperm analyzer (CASA) were used to evaluate the sperm motility and viability of post-thaw sperm. Results showed that the bull sperm motility and viability at the bottom of tubing-type canister was statistically higher and significant as compared to the commercial canister (p<0.05). Significant changes were found in sperm kinetics (VCL, VAP, VSL) of tubing-type canister compared to commercial canister. No significant changes in the motility and viability of the bull sperm at the top of tubing-type canister and commercial canister. There were no significant changes in sperm progression (LIN, WOB, PROG) in both the canisters. Developed tubing-type canister in this study showed potential as an alternative to be used in bull sperm cryo-storage |
| Disciplines: | Biología, Medicina veterinaria y zootecnia |
| Keyword: | Biología celular, Zootecnia, Biotecnología, Criopreservación, Esperma, Canister, Motilidad, Viabilidad |
| Keyword: | Biology, Veterinary medicine and animal husbandry, Cell biology, Animal husbandry, Biotechnology, Cryopreservation, Sperm, Canister, Motility, Viability |
| Full text: | Texto completo (Ver PDF) |
