| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000392685 |
| ISSN: | 1516-8913 |
| Authors: | Kumar, Ashwani1 Goyal, Suresh Chand1 Dhansu, Pooja1 Sheoran, Parvender2 Kumar, Arvind2 Verma, Ekta3 Parshad, Jagdish4 Singh, Anshuman2 |
| Institutions: | 1Haryana Agricultural University, Department of Botany and Plant Physiology, Hisar, Haryana. India 2Indian Council of Agricultural Research, Central Soil Salinity Research Institute, Hisar, Haryana. India 3Haryana Agricultural University, Department of Biochemistry, Hisar, Haryana. India 4Haryana Agricultural University, Department of Microbiology, Hisar, Haryana. India |
| Year: | 2015 |
| Season: | Sep-Oct |
| Volumen: | 58 |
| Number: | 5 |
| Pages: | 667-675 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | The present investigation was aimed to detect the specific polypeptide(s) appeared during the sequential stages of differentiation. Among different explants, only nodal explants showed good results for callusing. Depending on the fresh and dry weight, best callus growth was observed on MS medium supplemen ted with NAA (2.5 mg/L) in Dioscorea alata and 2, 4 - D (2.0 mg/L) in D. deltoidea , respectively. This callus was used for the regeneration. Roots differentiation was observed on MS medium + NAA (2.0 mg/L) + IBA (0.5 mg/L) and shoots on MS medium + BAP (2.0 mg/L) + NAA (0.5 mg/L) in D. alata while in D. deltoidea, roots on RT medium + IAA (1.0 mg/L) and shoots on RT medium + BAP (1.0 mg/L) + NAA (0.5 mg/L). Continuous decrease was seen in the total soluble protein during the differentiation in D. alata wherea s in D. deltoidea , the protein content decreased upto initiation stage. Four root specific polypeptides (MW 25.56, 24.35, 19.13 and 18.2 kDa) and three shoot specific polypeptides (MW 53.7, 25.12 and 19.13 kDa) were synthesized during the differentiation i n D. alata . Similarly, two root specific (MW 33.9 and 31.69 kDa) and one shoot specific (MW 16.98 kDa) polypeptide band were appeared during differentiation in D. deltoidea |
| Disciplines: | Biología, Agrociencias |
| Keyword: | Biología celular, Fitotecnia, Bioquímica, Dioscorea alata, Dioscorea deltoidea, Callos, Diferenciación, Polipéptidos, Biotecnología |
| Keyword: | Biology, Agricultural sciences, Cell biology, Crop husbandry, Biochemistry, Dioscorea alata, Dioscorea deltoidea, Callus, Differentiation, Polypeptides, Biotechnology |
| Full text: | Texto completo (Ver PDF) |
