| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000387716 |
| ISSN: | 1516-8913 |
| Authors: | Zanoni, Jacqueline Nelisis1 Hermes-Uliana, Catchia2 |
| Institutions: | 1Universidade Estadual de Maringa, Departamento de Ciencias Morfologicas, Maringa, Parana. Brasil 2Universidade Federal de Mato Grosso do Sul, Coxim, Mato Grosso do Sul. Brasil |
| Year: | 2015 |
| Season: | Jul-Ago |
| Volumen: | 58 |
| Number: | 4 |
| Pages: | 504-511 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | The present study evaluated the effects of supplementation with a combination of vitamin C and vitamin E on NADH-diaphorase-positive (NADH-d+) and neuronal nitric oxide synthase (nNOS)-immunoreactive myenteric neurons in the duodenum and ileum in diabetic rats. Forty rats were distributed into the following groups: normoglycemic (N), normoglycemic supplemented with vitamin C and vitamin E (NS), diabetic (D), and diabetic supplemented with vitamin C and vitamin E (DS). Vitamin C was added to the drinking water, and vitamin E was incorporated in the diet (1%). After 120 days, the animals were euthanized, and the duodenum and ileum were subjected to NADH-d and nNOS staining. Quantitative and morphometric analyses of myenteric neurons were performed. Diabetes reduced NADH-d+ neurons in the D group. The density of nitrergic neurons was not changed by diabetes or vitamin treatment. Hypertrophy of the cell body area of NADH-d+ and nNOS-immunoreactive neurons was observed in both intestinal segments. Combined supplementation with vitamin C and vitamin E prevented the reduction of the density of NADH-d+ neurons and hypertrophy, demonstratred by both techniques. Supplementation with a combination of vitamin C and vitamin E promoted myenteric neuroprotection in the small intestine in diabetic rats |
| Disciplines: | Medicina |
| Keyword: | Farmacología, Medicina experimental, Metabolismo y nutrición, Acido ascórbico, Alfa-tocoferol, Diabetes, Neuropatía diabética, Plexo mientérico, Ratas |
| Keyword: | Medicine, Experimental medicine, Metabolism and nutrition, Pharmacology, Ascorbic acid, Alpha-tocopherol, Diabetes, Diabetic neuropathy, Myenteric plexus, Rats |
| Full text: | Texto completo (Ver HTML) |
