| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000392820 |
| ISSN: | 1516-8913 |
| Authors: | Guelfi, Marieli1 Maioli, Marcos Antonio2 Tavares, Marco Aurelio1 Mingatto, Fabio Erminio1 |
| Institutions: | 1Universidade Estadual Paulista "Julio de Mesquita Filho", Departamento de Zootecnia, Dracena, Sao Paulo. Brasil 2Universidade Estadual Paulista "Julio de Mesquita Filho", Departamento de Producao e Saude Animal, Aracatuba, Sao Paulo. Brasil |
| Year: | 2015 |
| Season: | Nov-Dic |
| Volumen: | 58 |
| Number: | 6 |
| Pages: | 843-853 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | The aim of this study was to characterize the mechanism of toxicity of fipronil on hepatocytes isolated from the rat and the effect of its biotransformation on the toxicological potential. Th e toxicity of fipronil was assessed by monitoring the oxygen consumption and mitochondrial membrane potential, intracellular ATP concentration, Ca 2+ homeostasis and cell viability. The cell viability was evaluated by trypan blue exclusion in hepatocytes th at were isolated from the normal rats and by the release of the enzymes alanine transaminase and aspartate transaminase in hepatocytes that were isolated from the normal rats or proadifen - pretreated rats. Fipronil reduced mitochondrial respiration in the c ells that were energized with glutamate plus malate in a dose - dependent manner and dissipated the mitochondrial membrane potential that was accompanied by a reduction in ATP concentration and a disruption of intracellular Ca 2+ homeostasis. The cell viabili ty was affected by fipronil with higher potency in hepatocytes that were isolated from the normal rats, which indicated that the metabolism of this insecticide increased its toxicological potential. The results of this study indicated that the toxicity of fipronil to the hepatocytes was related to the inhibition of mitochondrial activity, which led to decreased ATP synthesis and a consequent alteration in intracellular Ca 2+ homeostasis and ultimately resulted in cell death |
| Disciplines: | Medicina, Química |
| Keyword: | Gastroenterología, Medicina experimental, Química farmacéutica, Fipronil, Hepatotoxicidad, Bioenergética, Biotransformación, Homeostasis, Calcio |
| Keyword: | Medicine, Chemistry, Experimental medicine, Gastroenterology, Medicinal chemistry, Fipronil, Hepatotoxicity, Bioenergetics, Biotransformation, Homeostasis, Calcium |
| Full text: | Texto completo (Ver PDF) |
