| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000374382 |
| ISSN: | 1516-8913 |
| Authors: | Rodrigues, Marcelo1 Festucci-Buselli, Reginaldo Alves2 Silva, Luzimar Campos3 Otoni, Wagner Campos3 |
| Institutions: | 1Universidade Federal de Lavras, Departamento de Biologia, Lavras, Minas Gerais. Brasil 2Universidade Federal Rural da Amazonia, Centro de Tecnologia Agropecuaria, Belem, Para. Brasil 3Universidade Federal de Vicosa, Departamento de Biología Vegetal, Vicosa, Minas Gerais. Brasil |
| Year: | 2014 |
| Season: | Mar-Abr |
| Volumen: | 57 |
| Number: | 2 |
| Pages: | 155-162 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental |
| English abstract | The use of cell and plant tissues culture techniques to produce economically important active metabolites has been growing. Among these substances, azadirachtin (AZA), produced by the neem tree (Azadirachta indica), has received considerable attention due to its bioinsecticide action. The main goal of this work was to analyze the AZA levels in neem cotyledonary calli. The calli were grown in agitated Woody Plant Medium (WPM) liquid medium, supplemented with glucose (Gl), hydrolyzed casein (HC) and methyl jasmonate (MeJ) as elicitor agent. An interaction was observed between these substances, depending on in vitro cultivation time with orbital agitation. The highest concentrations (average of 0.2470 µg g-1) of AZA were produced in the first and second weeks of culture when the cell mass was grown in a medium with 2% Gl v/v, 500 mg L-1 HC and 100 µM of MeJ. This corresponded to approximately 57% of the AZA content stored in the donor plants seeds, used as a source of explants to induce in vitro callus formation. It was concluded that the nutrition, as well as the concentration of MeJ as signal transduction of secondary metabolism in neem cells, might influence the AZA content produced in vitro |
| Disciplines: | Biología, Química |
| Keyword: | Biología celular, Bioquímica, Química analítica, Neem, Análisis por CLAE, Glucosa, Caseína hidrolizada, Jasmonato de metilo, Biotecnología |
| Keyword: | Biology, Chemistry, Cell biology, Analytical chemistry, Biochemistry, Neem, HPLC analysis, Glucose, Hydrolyzed casein, Methyl jasmonate, Biotechnology |
| Full text: | Texto completo (Ver PDF) |
