| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000365562 |
| ISSN: | 1516-8913 |
| Authors: | Fraige, Karina1 Travensolo, Regiane Fatima1 Carrilho, Emanuel1 |
| Institutions: | 1Universidade de Sao Paulo, Instituto de Quimica de Sao Carlos, Sao Carlos, Sao Paulo. Brasil |
| Year: | 2013 |
| Season: | Mar-Abr |
| Volumen: | 56 |
| Number: | 2 |
| Pages: | 213-222 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | The aim of this work was to evaluate two paternity cases by microchip electrophoresis and the validation of the methodology by comparison of the results with those obtained in a commercial genetic analyzer. It was observed that when working with tetranucleotide regions, in which the minimal difference between the alleles was only four base pairs, the commercial microchip system did not present the resolution and repeatability needed. Nevertheless, the relative standard deviation was between 0 and 1.2% and the fragments detected were within the expected size ranges as described in the literature |
| Disciplines: | Medicina |
| Keyword: | Genética, Medicina forense, Pruebas de paternidad, Reacción en cadena de la polimerasa (PCR), Loci, Secuencia génica |
| Keyword: | Medicine, Genetics, Forensic medicine, Paternity tests, Polymerase chain reaction (PCR), Loci, Gene sequence |
| Full text: | Texto completo (Ver HTML) |
