| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000374440 |
| ISSN: | 1516-8913 |
| Authors: | Trombete, Felipe Machado1 Castro, Izabela Miranda de2 Teixeira, Alessandra da Silva2 Saldanha, Tatiana1 Fraga, Marcelo Elias3 |
| Institutions: | 1Universidade Federal Rural do Rio de Janeiro, Departamento de Tecnologia de Alimentos, Seropedica, Rio de Janeiro. Brasil 2Empresa Brasileira de Pesquisa Agropecuaria, Agroindustria de Alimentos, Rio de Janeiro. Brasil 3Universidade Federal Rural do Rio de Janeiro, Departamento de Microbiologia e Imunologia Veterinaria, Seropedica, Rio de Janeiro. Brasil |
| Year: | 2014 |
| Season: | Mar-Abr |
| Volumen: | 57 |
| Number: | 2 |
| Pages: | 269-273 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental |
| English abstract | This study aimed to determine the occurrence of AFM1 contamination in the samples of grated parmesan cheese marketed in the Metropolitan Region of Rio de Janeiro -Brazil. Thirty samples representing 10 major brands marketed in the region were analyzed by High Performance Liquid Chromatography with fluorescence detection (HPLC-FLD) after purification with immunoaffinity column. The method showed recovery values within the range of 70-90%, with RSD lower than 15% and limits of detection and quantification below the maximum level allowed by the European Commission for the presence of AFM1 in cheeses. The mycotoxin was identified in 18 (60%) of the grated cheese samples tested. The highest value corresponded to 0.69 ± 0.02 µg/kg and the mean for all the analyzed samples was 0.16 µg/kg. All the samples were lower than the limit established by the Brazilian legislation (2.5 µg/kg) for AFM1 in cheeses in general. However, eight samples (26.7%) presented AFM1 levels above the tolerance limit of 0.25 µg/kg adopted by the European Commission. These results indicated that AFM1 levels in the grated cheese consumed in Rio de Janeiro -Brazil were relatively high and it could provide a potential hazard for the public health |
| Disciplines: | Química, Biología |
| Keyword: | Química analítica, Química de alimentos, Hongos, Micotoxinas, Productos lácteos, Cromatografía líquida de alta resolución (HPLC), Detector fluorescente (FLD) |
| Keyword: | Chemistry, Biology, Analytical chemistry, Food chemistry, Fungi, Mycotoxins, Dairy products, High performance liquid chromatography (HPLC), Fluorescence detector (FLD) |
| Full text: | Texto completo (Ver PDF) |
